ArticleNucleic acids research2011
Secondary structure is required for 3' splice site recognition in yeast.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Article
- Control of gene output by intron RNA structure.bioRxiv : the preprint server for biology · 2025Article
- Autoregulation of RPL7B by inhibition of a structural splicing enhancer.Nucleic acids research · 2025Article
- Co-transcriptional gene regulation in eukaryotes and prokaryotes.Nature reviews. Molecular cell biology · 2024Review
- Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons.Nucleic acids research · 2024Article
- Impact on splicing inRNA (New York, N.Y.) · 2023Article
- Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.Nucleic acids research · 2023Article
- Article
- How does precursor RNA structure influence RNA processing and gene expression?Bioscience reports · 2023Review
- Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.Nucleic acids research · 2022Article
- Group II Introns: Flexibility and Repurposing.Frontiers in molecular biosciences · 2022Article
- Improved recognition of splice sites in3 Biotech · 2021Article
- Reciprocal hemizygosity analysis reveals that the Saccharomyces cerevisiae CGI121 gene affects lag time duration in synthetic grape must.G3 (Bethesda, Md.) · 2021Article
- Article
- Evolution of intron splicing towards optimized gene expression is based on various Cis- and Trans-molecular mechanisms.PLoS biology · 2019Article
- RNAs in the spliceosome: Insight from cryoEM structures.Wiley interdisciplinary reviews. RNA · 2019Review
- Introns provide a platform for intergenic regulatory feedback of RPL22 paralogs in yeast.PloS one · 2018Article
- Structure of the yeast spliceosomal postcatalytic P complex.Science (New York, N.Y.) · 2017Article
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- The effects of structure on pre-mRNA processing and stability.Methods (San Diego, Calif.) · 2017Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition.
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