ArticleExperimental parasitology2011
Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis.
Article in Experimental parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Serum antibody screening using glycan arrays.Chemical Society reviews · 2024Review
- Glycan Array Technology.Advances in biochemical engineering/biotechnology · 2021Article
- Comparisons of N-glycans across invertebrate phyla.Parasitology · 2019Review
- Highly modified and immunoactive N-glycans of the canine heartworm.Nature communications · 2019Article
- Immunomodulation by Helminths: Intracellular Pathways and Extracellular Vesicles.Frontiers in immunology · 2018Review
- Chagas Disease Diagnostic Applications: Present Knowledge and Future Steps.Advances in parasitology · 2017Review
- Serological tools for detection ofOne health (Amsterdam, Netherlands) · 2016Review
- Novel O-linked methylated glycan antigens decorate secreted immunodominant glycoproteins from the intestinal nematode Heligmosomoides polygyrus.International journal for parasitology · 2016Article
- Factors Affecting Anti-Glycan IgG and IgM Repertoires in Human Serum.Scientific reports · 2016Article
- Article
- Differential expression of anti-glycan antibodies in schistosome-infected humans, rhesus monkeys and mice.Glycobiology · 2014Article
- Immunization with recombinantly expressed glycan antigens from Schistosoma mansoni induces glycan-specific antibodies against the parasite.Glycobiology · 2014Article
- Array-assisted characterization of a fucosyltransferase required for the biosynthesis of complex core modifications of nematode N-glycans.The Journal of biological chemistry · 2013Article
- Carbohydrate microarrays.Chemical Society reviews · 2013Review
- Glycoconjugates in host-helminth interactions.Frontiers in immunology · 2013Review
- Review
- A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases.PLoS neglected tropical diseases · 2012Article
- Plasticity of the β-trefoil protein fold in the recognition and control of invertebrate predators and parasites by a fungal defence system.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Diagnostic methods for parasite infections still highly depend on the identification of the parasites by direct methods such as microscopic examination of blood, stool and tissue biopsies. Serodiagnosis is often carried out to complement the direct methods; however, few synthetic antigens with sufficient sensitivity and specificity are available. Here we evaluated a glycan microarray approach to select for synthetic glycan antigens that could be used for serodiagnosis of parasitic infections. Using a glycan array containing over 250 different glycan antigens, we identified GalNAcβ1-4(Fucα1-3)GlcNAc-R (LDNF) as a glycan antigen that is recognized by antibodies from Trichinella-infected individuals. We synthesized a neoglycoconjugate, consisting of five LDNF molecules covalently coupled to bovine serum albumin (BSA), and used this neoglycoconjugate as an antigen to develop a highly sensitive total-Ig ELISA for serological screening of trichinellosis. The results indicate that glycan microarrays constitute a promising technology for fast and specific identification of parasite glycan antigens to improve serodiagnosis of different parasitic infections, either using an ELISA format, or parasite-specific glycan arrays.
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Registered trials
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