ArticlePflugers Archiv : European journal of physiology2011
Calcium-dependent decrease in the single-channel conductance of TRPV1.
Article in Pflugers Archiv : European journal of physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- TRPV1 and TRPA1 channels exhibit bifurcated sensing of singlet oxygen and hydrogen peroxide.Redox biology · 2026Article
- In silico analysis of sodium ion permeation mechanisms in transient receptor potential vanilloid 1.Scientific reports · 2025Article
- ROS-induced voltage-gated ion channel expression and electrophysiological remodeling in malignant human cells.NPJ systems biology and applications · 2025Article
- Permeant cations modulate pore dynamics and gating of TRPV1 ion channels.The Journal of general physiology · 2024Article
- Calcium binding and permeation in TRPV channels: Insights from molecular dynamics simulations.The Journal of general physiology · 2023Article
- The Transient Receptor Potential Vanilloid 2 (TRPV2) Channel Facilitates Virus Infection Through the CaAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- The Calcium Signaling Mechanisms in Arterial Smooth Muscle and Endothelial Cells.Comprehensive Physiology · 2021Article
- Article
- Recovery from tachyphylaxis of TRPV1 coincides with recycling to the surface membrane.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Different agonists induce distinct single-channel conductance states in TRPV1 channels.The Journal of general physiology · 2018Article
- Direct Anandamide Activation of TRPV1 Produces Divergent Calcium and Current Responses.Frontiers in molecular neuroscience · 2017Article
- Measurement of relative Ca²⁺ permeability during sustained activation of TRPV1 receptors.Pflugers Archiv : European journal of physiology · 2016Article
- Membrane Protein Properties Revealed through Data-Rich Electrostatics Calculations.Structure (London, England : 1993) · 2015Article
- Divalent cations potentiate TRPV1 channel by lowering the heat activation threshold.The Journal of general physiology · 2014Article
- Principles and properties of ion flow in P2X receptors.Frontiers in cellular neuroscience · 2014Review
- TRPV1 and SP: key elements for sepsis outcome?British journal of pharmacology · 2013Review
- Gastrin-releasing peptide acts via postsynaptic BB2 receptors to modulate inward rectifier K+ and TRPV1-like conductances in rat paraventricular thalamic neurons.The Journal of physiology · 2013Article
- Activation and desensitization of TRPV1 channels in sensory neurons by the PPARα agonist palmitoylethanolamide.British journal of pharmacology · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
TRPV1 is a Ca(2+) permeable cation channel gated by multiple stimuli including noxious heat, capsaicin, protons, and extracellular cations. In this paper, we show that Ca(2+) causes a concentration and voltage-dependent decrease in the capsaicin-gated TRPV1 single-channel conductance. This Ca(2+)-dependent effect on conductance was strongest at membrane potentials between -60 and +20 mV, but was diminished at more hyperpolarised potentials. Using simultaneous recordings of membrane current and fura-2 fluorescence to measure the fractional Ca(2+) current of whole-cell currents evoked through wild-type and mutant TRPV1, we investigated a possible link between the mechanisms underlying Ca(2+) permeation and the Ca(2+)-dependent effect on conductance. Surprisingly, we found no evidence of a structural correlation, and observed that the substitution of amino acids known to regulate Ca(2+) permeability had little effect on the ability for Ca(2+) to decrease TRPV1 conductance. However, we did observe that the Ca(2+)-dependent effect on conductance was not diminished by negative hyperpolarisation for a mutant receptor with severely impaired Ca(2+) permeability, TRPV1-D646N/E648Q/E651Q. This would be consistent with the idea that Ca(2+) reduces conductance by interacting with an intra-pore binding site, and that negative hyperpolarization reduces occupancy of this site by speeding the exit of Ca(2+) into the cell. Taken together, our data show that in addition to directly and indirectly regulating channel gating, Ca(2+) also directly reduces the conductance of TRPV1. Surprisingly, the mechanism underlying this Ca(2+)-dependent effect on conductance is largely independent of mechanisms governing Ca(2+) permeability.
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Registered trials
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