ArticleStem cell reviews and reports2012
Two- and three-dimensional culture of keratinocyte stem and precursor cells derived from primary murine epidermal cultures.
Article in Stem cell reviews and reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- The evolving landscape of eyebrow reconstruction: from traditional flaps to minimally invasive techniques and regenerative medicine.Frontiers in surgery · 2026Review
- From basic biology to engineered therapies: the keratinocyte stem cell playbook.Frontiers in medical technology · 2026Review
- Combining advanced 3D spheroid-based skin models with deep-learning-based image analysis enables in-depth investigation of keratinocyte differentiation and barrier function.Frontiers in bioengineering and biotechnology · 2026Article
- JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes.Histochemistry and cell biology · 2024Article
- Article
- Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.Microscopy (Oxford, England) · 2022Article
- Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity.Journal of visualized experiments : JoVE · 2021Article
- Self-assembling 3D spheroid cultures of human neonatal keratinocytes have enhanced regenerative properties.Stem cell research · 2020Article
- Anisomycin, a JNK and p38 activator, suppresses cell-cell junction formation in 2D cultures of K38 mouse keratinocyte cells and reduces claudin-7 expression, with an increase of paracellular permeability in 3D cultures.Histochemistry and cell biology · 2019Article
- A Novel Fully Humanized 3D Skin Equivalent to Model Early Melanoma Invasion.Molecular cancer therapeutics · 2015Article
- Epidermal keratinocytes initiate wound healing and pro-inflammatory immune responses following percutaneous schistosome infection.International journal for parasitology · 2015Article
- Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potential.Cell death and differentiation · 2014Article
- Rapid isolation of integrin rich multipotent stem cell pool and reconstruction of mouse epidermis equivalent.American journal of stem cells · 2014Article
- The c-Rel subunit of NF-κB regulates epidermal homeostasis and promotes skin fibrosis in mice.The American journal of pathology · 2013Article
- Epidermal stem cells in orthopaedic regenerative medicine.International journal of molecular sciences · 2013Review
- Article
- Highly efficient zinc-finger nuclease-mediated disruption of an eGFP transgene in keratinocyte stem cells without impairment of stem cell properties.Stem cell reviews and reports · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the skin, multipotent keratinocyte stem cells (KSC) are localised in the hair follicle bulge region. Although, KSC can be cultivated and grown in two-dimensional (2D) culture they rapidly lose stem cell markers when isolated from their niche. Currently, there is no KSC culture method available which recapitulates an environment similar to the KSC niche in the hair follicle. Here we describe the successful establishment of an in vitro 3D stem cell culture model developed from clonally growing keratinocyte lines derived from neonatal mice using culture conditions previously established for human keratinocytes. After 20 passages, keratinocyte lines showed a stable ratio of holoclones (stem cells), meroclones (stem and precursor cells) and paraclones (differentiating cells), with approximately 29% holoclones, 54% meroclones and 17% paraclones, and were thus termed keratinocyte stem and precursor cell (KSPC) cultures. In high calcium medium, KSPC cultures grown at the air-liquid interphase differentiated and formed epidermal equivalents. Notably, and in contrast to primary keratinocytes, keratinocytes from KSPC cultures were able to aggregate and form spherical clusters in hanging drops, a characteristic hallmark shared with other stem cell types. Similar to the in vivo situation in the hair follicle bulge, KSPC aggregates also showed low proliferation, down-regulation of keratin 6, absence of keratin 1, and expression of the KSC markers keratin 15, Sox9, NFATc1 and Zfp145. KSPC aggregates therefore provide an optimal in vitro 3D environment for the further characterisation and study of normal and genetically modified KSPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.