ArticleNucleic acids research2011
Complexity in the binding of minor groove agents: netropsin has two thermodynamically different DNA binding modes at a single site.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Novel guanidine derivatives as mechanistic scaffolds for anticancer agents: synthesis, characterization, DNA-binding, and computational studies.RSC advances · 2025Article
- Synthesis and Molecular Dynamic Simulation of Novel Cationic and Non-cationic Pyrimidine Derivatives as Potential G-quadruplex-ligands.Anti-cancer agents in medicinal chemistry · 2024Article
- DNA major versus minor groove occupancy of monomeric and dimeric crystal violet derivatives. Toward structural correlations.Bioorganic & medicinal chemistry · 2023Article
- Modular Synthesis of Highly Substituted 3-Azapyrroles by Rh(II)-Catalyzed N-H Bond Insertion and Cyclodehydration.The Journal of organic chemistry · 2022Article
- Examining the Effects of Netropsin on the Curvature of DNA A-Tracts Using Electrophoresis.Molecules (Basel, Switzerland) · 2021Article
- Nonhost Disease Resistance in Pea: Chitosan's Suggested Role in DNA Minor Groove Actions Relative to Phytoalexin-Eliciting Anti-Cancer Compounds.Molecules (Basel, Switzerland) · 2020Article
- Anticancer potential of metabolic compounds from marine actinomycetes isolated from Lagos Lagoon sediment.Journal of pharmaceutical analysis · 2019Article
- Accurate Estimation of the Standard Binding Free Energy of Netropsin with DNA.Molecules (Basel, Switzerland) · 2018Article
- Imino proton NMR guides the reprogramming of A•T specific minor groove binders for mixed base pair recognition.Nucleic acids research · 2016Article
- Modeling complex equilibria in isothermal titration calorimetry experiments: thermodynamic parameters estimation for a three-binding-site model.Analytical biochemistry · 2013Article
- Cooperative stabilization of Zn(2+):DNA complexes through netropsin binding in the minor groove of FdU-substituted DNA.Journal of biomolecular structure & dynamics · 2013Article
- Studies on the interaction mechanism of pyrene derivatives with human tumor-related DNA.Molecules (Basel, Switzerland) · 2012Article
- Melting thermodynamics of reversible DNA/ligand complexes at interfaces.Journal of the American Chemical Society · 2012Article
- Microscopic rearrangement of bound minor groove binders detected by NMR.The journal of physical chemistry. B · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Structural results with minor groove binding agents, such as netropsin, have provided detailed, atomic level views of DNA molecular recognition. Solution studies, however, indicate that there is complexity in the binding of minor groove agents to a single site. Netropsin, for example, has two DNA binding enthalpies in isothermal titration calorimetry (ITC) experiments that indicate the compound simultaneously forms two thermodynamically different complexes at a single AATT site. Two proposals for the origin of this unusual observation have been developed: (i) two different bound species of netropsin at single binding sites and (ii) a netropsin induced DNA hairpin to duplex transition. To develop a better understanding of DNA recognition complexity, the two proposals have been tested with several DNAs and the methods of mass spectrometry (MS), polyacrylamide gel electrophoresis (PAGE) and nuclear magnetic resonance spectroscopy in addition to ITC. All of the methods with all of the DNAs investigated clearly shows that netropsin forms two different complexes at AATT sites, and that the proposal for an induced hairpin to duplex transition in this system is incorrect.
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