ArticleNucleic acids research2011
Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Review
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- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.bioRxiv : the preprint server for biology · 2023Article
- Comparative analysis reveals distinctive genomic features of Taiwan hot-spring cyanobacteriumFrontiers in microbiology · 2022Article
- Comparative Genomics of Three Novel Jumbo Bacteriophages Infecting Staphylococcus aureus.Journal of virology · 2021Article
- F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif.Mobile DNA · 2018Article
- Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNA.Nucleic acids research · 2014Article
- Resilience of biochemical activity in protein domains in the face of structural divergence.Current opinion in structural biology · 2014Review
- Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Generation of gene-modified mice via Cas9/RNA-mediated gene targeting.Cell research · 2013Article
- Romulus and Remus, two phage isolates representing a distinct clade within the Twortlikevirus genus, display suitable properties for phage therapy applications.Journal of virology · 2013Article
- Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease.Nucleic acids research · 2013Article
- A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.Science (New York, N.Y.) · 2012Article
- Sequence, structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily.Nucleic acids research · 2012Article
- Structural, functional and evolutionary relationships between homing endonucleases and proteins from their host organisms.Nucleic acids research · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
Novel family of putative homing endonuclease genes was recently discovered during analyses of metagenomic and genomic sequence data. One such protein is encoded within a group I intron that resides in the recA gene of the Bacillus thuringiensis 03058-36 bacteriophage. Named I-Bth0305I, the endonuclease cleaves a DNA target in the uninterrupted recA gene at a position immediately adjacent to the intron insertion site. The enzyme displays a multidomain, homodimeric architecture and footprints a DNA region of ~60 bp. Its highest specificity corresponds to a 14-bp pseudopalindromic sequence that is directly centered across the DNA cleavage site. Unlike many homing endonucleases, the specificity profile of the enzyme is evenly distributed across much of its target site, such that few single base pair substitutions cause a significant decrease in cleavage activity. A crystal structure of its C-terminal domain confirms a nuclease fold that is homologous to very short patch repair (Vsr) endonucleases. The domain architecture and DNA recognition profile displayed by I-Bth0305I, which is the prototype of a homing lineage that we term the 'EDxHD' family, are distinct from previously characterized homing endonucleases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.