ArticleGenetics2011
Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Constructing an efficient synthesis pathway in peroxisomes to boostSynthetic and systems biotechnology · 2026Article
- A double role of the Gal80 N terminus in activation of transcription by Gal4p.Life science alliance · 2020Article
- Multi-component gene network design as a survival strategy in diverse environments.BMC systems biology · 2018Article
- Assigning function to natural allelic variation via dynamic modeling of gene network induction.Molecular systems biology · 2018Article
- Noise reduction as an emergent property of single-cell aging.Nature communications · 2017Article
- The 9aaTAD Is Exclusive Activation Domain in Gal4.PloS one · 2017Article
- Reverse engineering gene regulatory networks from measurement with missing values.EURASIP journal on bioinformatics & systems biology · 2016Article
- Noise reduction facilitated by dosage compensation in gene networks.Nature communications · 2016Article
- Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.Molecular and cellular biology · 2015Article
- Yeast Replicator: A High-Throughput Multiplexed Microfluidics Platform for Automated Measurements of Single-Cell Aging.Cell reports · 2015Article
- Allelic variation, aneuploidy, and nongenetic mechanisms suppress a monogenic trait in yeast.Genetics · 2015Article
- The yeast galactose network as a quantitative model for cellular memory.Molecular bioSystems · 2015Review
- Long noncoding RNAs promote transcriptional poising of inducible genes.PLoS biology · 2013Article
- Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.Molecular and cellular biology · 2013Article
- Coupling of septins to the axial landmark by Bud4 in budding yeast.Journal of cell science · 2013Article
- Inference of gene regulatory networks from genome-wide knockout fitness data.Bioinformatics (Oxford, England) · 2013Article
- Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes.Eukaryotic cell · 2012Article
- The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.Genes & development · 2012Article
- N-acetylglucosamine (GlcNAc) functions in cell signaling.Scientifica · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The yeast transcriptional activator Gal4 localizes to UAS(GAL) sites even in the absence of galactose but cannot activate transcription due to an association with the Gal80 protein. By 4 min after galactose addition, Gal4-activated gene transcription ensues. It is well established that this rapid induction arises through a galactose-triggered association between the Gal80 and Gal3 proteins that decreases the association of Gal80 and Gal4. How this happens mechanistically remains unclear. Strikingly different hypotheses prevail concerning the possible roles of nucleocytoplasmic distribution and trafficking of Gal3 and Gal80 and where in the cell the initial Gal3-Gal80 association occurs. Here we tested two conflicting hypotheses by evaluating the subcellular distribution and dynamics of Gal3 and Gal80 with reference to induction kinetics. We determined that the rates of nucleocytoplasmic trafficking for both Gal80 and Gal3 are slow relative to the rate of induction. We find that depletion of the nuclear pool of Gal3 slows the induction kinetics. Thus, nuclear Gal3 is critical for rapid induction. Fluorescence-recovery-after-photobleaching experiments provided data suggesting that the Gal80-Gal4 complex exhibits kinetic stability in the absence of galactose. Finally, we detect Gal3 at the UAS(GAL) only if Gal80 is covalently linked to the DNA-binding domain. Taken altogether, these new findings lead us to propose that a transient interaction of Gal3 with Gal4-associated Gal80 could explain the rapid response of this system. This notion could also explain earlier observations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.