Evidence map›Paper›PMID 21889511›Full record

ArticleJournal of immunological methods2011

An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes.

Aleah L Brubaker, David F Schneider, Jessica L Palmer, Douglas E Faunce, Elizabeth J Kovacs

Abstract read
In one paragraph

Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 17 citations in OpenAlex.

  1. Phagocytosis of apoptotic endothelial cells reprograms macrophages in skin wounds.Journal of immunology and regenerative medicine · 2021
    Article
  2. LPSFrontiers in immunology · 2021
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Mice engrafted with human hematopoietic stem cells support a human myeloid cell inflammatory response in vivo.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2016
    Article
  11. Article
  12. Experimental Approaches to Tissue Injury and Repair in Advanced Age.Methods in molecular biology (Clifton, N.J.) · 2015
    Article
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Aleah L BrubakerThe Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA.
David F Schneider
Jessica L Palmer
Douglas E Faunce
Elizabeth J Kovacs
Loyola University Medical Center · US

Funding

Aging, macrophage mediators, and burn trauma: SupplementR01AG018859 · NIA · UNIVERSITY OF COLORADO DENVER · PI KOVACS, ELIZABETH J. · 2001 to 2024
$7.6M
Natural Killer T Cell Modulation of Cutaneous Wound HealingR21AI073987 · NIAID · LOYOLA UNIVERSITY CHICAGO · PI KOVACS, ELIZABETH J. · 2009 to 2010
$411k
Training in Immunology and AgingT32AG031780 · NIA · LOYOLA UNIVERSITY CHICAGO · PI WITTE, PAMELA L. · 2009 to 2010
$148k
NIAID NIH HHS R21 AI073987NIA NIH HHS R01 AG018859NIA NIH HHS T32 AG031780
6 · The paper itself

Abstract

Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.

Indexed as

AnimalsCells, CulturedCell SeparationFlow CytometryLeukocytesMicePhagocytosisSkinStaphylococcus aureus

Identifiers

PMID21889511
PMCPMC3195830
OpenAlexW2091964325

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.