ArticleBiophysical journal2011
Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- AFM-Fold: Rapid reconstruction of protein conformations from AFM images.Biophysical journal · 2026Article
- Supramolecular Assembly and Interfacial Hydration of Tandem Repeat Dipeptides on 2D Nanomaterials: Insights From 3D-AFM Measurements and MD Simulations.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Modeling Conformational Transitions of Biomolecules from Atomic Force Microscopy Images using Normal Mode Analysis.The journal of physical chemistry. B · 2024Article
- Protein nanoarrays using the annexin A5 two-dimensional crystal on supported lipid bilayers.Nanoscale advances · 2023Article
- Atomic force microscopic analysis of the light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum.Photosynthesis research · 2023Article
- Spatiotemporal resolution in high-speed atomic force microscopy for studying biological macromolecules in action.Microscopy (Oxford, England) · 2023Review
- Development of hidden Markov modeling method for molecular orientations and structure estimation from high-speed atomic force microscopy time-series images.PLoS computational biology · 2022Article
- Inferring Conformational State of Myosin Motor in an Atomic Force Microscopy ImageFrontiers in molecular biosciences · 2022Article
- When Order Meets Disorder: Modeling and Function of the Protein Interface in Fuzzy Complexes.Biomolecules · 2021Review
- Stretching the resolution limit of atomic force microscopy.Nature structural & molecular biology · 2021Article
- Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2.Scientific reports · 2019Article
- Article
- Immunoactivity of self-assembled antibodies investigated by atomic force microscopy.RSC advances · 2018Article
- Quantitative comparison of wideband low-latency phase-locked loop circuit designs for high-speed frequency modulation atomic force microscopy.Beilstein journal of nanotechnology · 2018Article
- Filming biomolecular processes by high-speed atomic force microscopy.Chemical reviews · 2014Review
- Computational reconstruction of multidomain proteins using atomic force microscopy data.Structure (London, England : 1993) · 2012Article
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6 authors.
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Abstract
In this study, we directly imaged subnanometer-scale structures of tubulins by performing frequency modulation atomic force microscopy (FM-AFM) in liquid. Individual α-helices at the surface of a tubulin protofilament were imaged as periodic corrugations with a spacing of 0.53 nm, which corresponds to the common pitch of an α-helix backbone (0.54 nm). The identification of individual α-helices allowed us to determine the orientation of the deposited tubulin protofilament. As a result, C-terminal domains of tubulins were identified as protrusions with a height of 0.4 nm from the surface of the tubulin. The imaging mechanism for the observed subnanometer-scale contrasts is discussed in relation to the possible structures of the C-terminal domains. Because the C-terminal domains are chemically modified to regulate the interactions between tubulins and other biomolecules (e.g., motor proteins and microtubule-associated proteins), detailed structural information on individual C-terminal domains is valuable for understanding such regulation mechanisms. The results obtained in this study demonstrate that FM-AFM is capable of visualizing the structural variation of tubulins with subnanometer resolution. This is an important first step toward using FM-AFM to analyze the functions of tubulins.
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