ArticleBiophysical journal2011
Rectification of the channelrhodopsin early conductance.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Pore-Opening and Ion-Conduction Mechanism in Channelrhodopsins C1C2, ChR2, and iChloC by Computational Electrophysiology and Constant-pH Simulations.Journal of chemical information and modeling · 2025Article
- Structural Insights Into the Opening Mechanism of C1C2 Channelrhodopsin.Journal of the American Chemical Society · 2025Article
- Potassium-selective channelrhodopsins.Biophysics and physicobiology · 2023Article
- The effect on ion channel of different protonation states of E90 in channelrhodopsin-2: a molecular dynamics simulation.RSC advances · 2021Article
- Emerging Diversity of Channelrhodopsins and Their Structure-Function Relationships.Frontiers in cellular neuroscience · 2021Review
- Nonlinear response characteristics of neural networks and single neurons undergoing optogenetic excitation.Network neuroscience (Cambridge, Mass.) · 2020Article
- Molecular Dynamics Simulation of Transmembrane Transport of Chloride Ions in Mutants of Channelrhodopsin.Biomolecules · 2019Article
- Formation Mechanism of Ion Channel in Channelrhodopsin-2: Molecular Dynamics Simulation and Steering Molecular Dynamics Simulations.International journal of molecular sciences · 2019Article
- Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents.The Journal of biological chemistry · 2019Article
- Site-specific ion occupation in the selectivity filter causes voltage-dependent gating in a viral KScientific reports · 2018Article
- Defining the ionic mechanisms of optogenetic control of vascular tone by channelrhodopsin-2.British journal of pharmacology · 2018Article
- Rhodopsin optogenetic toolbox v2.0 for light-sensitive excitation and inhibition in Caenorhabditis elegans.PloS one · 2018Article
- Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins.Journal of visualized experiments : JoVE · 2017Article
- Molecular properties of a DTD channelrhodopsin fromBiophysics and physicobiology · 2017Article
- An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels.eLife · 2016Article
- Proteomonas sulcata ACR1: A Fast Anion Channelrhodopsin.Photochemistry and photobiology · 2016Article
- PyRhO: A Multiscale Optogenetics Simulation Platform.Frontiers in neuroinformatics · 2016Article
- A simple recipe for setting up the flux equations of cyclic and linear reaction schemes of ion transport with a high number of states: The arrow scheme.Channels (Austin, Tex.) · 2016Article
- Gating mechanisms of a natural anion channelrhodopsin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analyzed the nonlinear current-voltage relationships of the early conducting state of channelrhodopsin-2 expressed in Xenopus oocytes and human embryonic kidney cells with respect to changes of the electrochemical gradients of H(+), Na(+)/K(+), and Ca(2+)/Mg(2+). Several models were tested for wild-type ChR2 and mutations at positions E90, E123, H134, and T159. Voltage-gating was excluded as cause for the nonlinearity. However, a general enzyme kinetic model with one predominant binding site yielded good fits throughout. The empty site with an apparent charge number of about -0.3 and strong external cation binding causes some inward rectification of the uniport function. Additional inward rectification is due to asymmetric competition from outside between the transported ion species. Significant improvement of the fits was achieved by introducing an elastic voltage-divider formed by the voltage-sensitive barriers.
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