ArticleAnnals of clinical microbiology and antimicrobials2011
Rapid PCR detection of group A Streptococcus from flocked throat swabs: a retrospective clinical study.
Article in Annals of clinical microbiology and antimicrobials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Rapid antigen detection test for group A streptococcus in children with pharyngitis.The Cochrane database of systematic reviews · 2016Pooled it
- An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis.BMC infectious diseases · 2021Trial
- Evaluation of a fully automated "sample-to-result" PCR-based diagnostic test for detecting A/C/G Streptococci from throat swab specimens.Microbiology spectrum · 2026Article
- Microbiological assessment of Suncus murinus bred and managed as laboratory animals.The Journal of veterinary medical science · 2024Article
- Acute Fulminant Group A Beta-Hemolytic Streptococcus-Associated Carditis: A Case Report and Literature Review.Cureus · 2022Article
- Antibody Modified Gold Electrode as an Impedimetric Biosensor for the Detection ofSensors (Basel, Switzerland) · 2020Article
- The use of bacterial DNA from saliva for the detection of GAS pharyngitis.Journal of oral microbiology · 2020Article
- High virulence gene diversity inPeerJ · 2019Article
- Simultaneous Detection of Nine Key Bacterial Respiratory Pathogens Using Luminex xTAGInternational journal of environmental research and public health · 2017Article
- Identification of Streptococcus pyogenes - Phenotypic Tests vs Molecular Assay (spy1258PCR): A Comparative Study.Journal of clinical and diagnostic research : JCDR · 2016Article
- Throat and nasal swabs for molecular detection of respiratory viruses in acute pharyngitis.Virology journal · 2015Article
- Detection of Streptococcus pyogenes by use of Illumigene group A Streptococcus assay.Journal of clinical microbiology · 2013Article
- Detection of group a streptococcal pharyngitis by quantitative PCR.BMC infectious diseases · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRapid diagnosis of GAS pharyngitis may improve patient care by ensuring that patients with GAS pharyngitis are treated quickly and also avoiding unnecessary use of antibiotics in those without GAS infection. Very few molecular methods for detection of GAS in clinical throat swab specimens have been described.
methodsWe performed a study of a laboratory-developed internally-controlled rapid Group A streptococcus (GAS) PCR assay using flocked swab throat specimens. We compared the GAS PCR assay to GAS culture results using a collection of archived throat swab samples obtained during a study comparing the performance of conventional and flocked throat swabs.
resultsThe sensitivity of the GAS PCR assay as compared to the reference standard was 96.0% (95% CI 90.1% to 98.4%), specificity 98.6% (95% CI 95.8% to 99.5%), positive predictive value (PPV) 96.9% (95% CI 91.4% to 99.0%) and negative predictive value (NPV) of 98.1% (95% CI 95.2% to 99.2%). For conventional swab cultures, sensitivity was 96.0% (95% CI 90.1% to 98.4%), specificity 100% (95% CI 98.2% to 100%), PPV 100%, (95% CI 96.1% to 100%) and NPV 98.1% (95% CI 95.2% to 99.3%)
conclusionsIn this retrospective study, the GAS PCR assay appeared to perform as well as conventional throat swab culture, the current standard of practice. Since the GAS PCR assay, including DNA extraction, can be performed in approximately 1 hour, prospective studies of this assay are warranted to evaluate the clinical impact of the assay on management of patients with pharyngitis.
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