ArticleParticle and fibre toxicology2011
In vitro toxicity of particulate matter (PM) collected at different sites in the Netherlands is associated with PM composition, size fraction and oxidative potential--the RAPTES project.
Article in Particle and fibre toxicology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
91 citing papers in PubMed, 3 syntheses or guidelines pooled it, 365 citations in OpenAlex.
- The short- and long-term associations of particulate matter with inflammation and blood coagulation markers: A meta-analysis.Environmental pollution (Barking, Essex : 1987) · 2020Pooled it
- Oxidative Potential Versus Biological Effects: A Review on the Relevance of Cell-Free/Abiotic Assays as Predictors of Toxicity from Airborne Particulate Matter.International journal of molecular sciences · 2019Pooled it
- Effect of particulate matter air pollution on C-reactive protein: a review of epidemiologic studies.Reviews on environmental health · 2012Pooled it
- Trial
- Assessment of the capacity of vehicle cabin air inlet filters to reduce diesel exhaust-induced symptoms in human volunteers.Environmental health : a global access science source · 2014Trial
- Composition of PM affects acute vascular inflammatory and coagulative markers - the RAPTES project.PloS one · 2013Trial
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- A Human Vocal Fold Organ-On-Chip for Studying Platform-Dependent Mucosal Responses to Particulate Matter.bioRxiv : the preprint server for biology · 2026Article
- Can oxidative potential be a plant risk indicator for heavy metals contaminated soil? Analysis of ryegrass (Eco-Environment & Health · 2025Article
- Differential inflammation, oxidative stress and cardiovascular damage markers of nano- and micro-particle exposure in mice: Implications for human disease burden.Redox biology · 2025Article
- Health risk and toxicity assessment of exhaust air from non-incineration devices for hospital waste treatment in Tehran, Iran.Scientific reports · 2025Article
- Causal relationships between air pollution and common autoimmune diseases: a two-sample Mendelian randomization study.Scientific reports · 2025Article
- The effects of photochemical aging and interactions with secondary organic aerosols on cellular toxicity of combustion particles.Journal of aerosol science · 2025Article
- Air Pollution and Osteoporosis.Current osteoporosis reports · 2024Review
- Assessing the Impact of Non-Exhaust Emissions on the Asthmatic Airway (IONA) Protocol for a Randomised Three-Exposure Crossover Study.International journal of environmental research and public health · 2024Article
- Inter-continental variability in the relationship of oxidative potential and cytotoxicity with PMNature communications · 2024Article
- The Oxidative Potential of Airborne Particulate Matter Research Trends, Challenges, and Future Perspectives-Insights from a Bibliometric Analysis and Scoping Review.Antioxidants (Basel, Switzerland) · 2024Article
- Coarse particulate matter (PM10) induce an inflammatory response through the NLRP3 activation.Journal of inflammation (London, England) · 2024Article
- Monitoring redox stress in human airway epithelial cells exposed to woodsmoke at an air-liquid interface.Particle and fibre toxicology · 2024Article
- Identifying urban emission sources and their contribution to the oxidative potential of fine particulate matter (PMEnvironmental pollution (Barking, Essex : 1987) · 2024Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundAmbient particulate matter (PM) exposure is associated with respiratory and cardiovascular morbidity and mortality. To what extent such effects are different for PM obtained from different sources or locations is still unclear. This study investigated the in vitro toxicity of ambient PM collected at different sites in the Netherlands in relation to PM composition and oxidative potential.
methodPM was sampled at eight sites: three traffic sites, an underground train station, as well as a harbor, farm, steelworks, and urban background location. Coarse (2.5-10 μm), fine (< 2.5 μm) and quasi ultrafine PM (qUF; < 0.18 μm) were sampled at each site. Murine macrophages (RAW 264.7 cells) were exposed to increasing concentrations of PM from these sites (6.25-12.5-25-50-100 μg/ml; corresponding to 3.68-58.8 μg/cm2). Following overnight incubation, MTT-reduction activity (a measure of metabolic activity) and the release of pro-inflammatory markers (Tumor Necrosis Factor-alpha, TNF-α; Interleukin-6, IL-6; Macrophage Inflammatory Protein-2, MIP-2) were measured. The oxidative potential and the endotoxin content of each PM sample were determined in a DTT- and LAL-assay respectively. Multiple linear regression was used to assess the relationship between the cellular responses and PM characteristics: concentration, site, size fraction, oxidative potential and endotoxin content.
resultsMost PM samples induced a concentration-dependent decrease in MTT-reduction activity and an increase in pro-inflammatory markers with the exception of the urban background and stop & go traffic samples. Fine and qUF samples of traffic locations, characterized by a high concentration of elemental and organic carbon, induced the highest pro-inflammatory activity. The pro-inflammatory response to coarse samples was associated with the endotoxin level, which was found to increase dramatically during a three-day sample concentration procedure in the laboratory. The underground samples, characterized by a high content of transition metals, showed the largest decrease in MTT-reduction activity. PM size fraction was not related to MTT-reduction activity, whereas there was a statistically significant difference in pro-inflammatory activity between Fine and qUF PM. Furthermore, there was a statistically significant negative association between PM oxidative potential and MTT-reduction activity.
conclusionThe response of RAW264.7 cells to ambient PM was markedly different using samples collected at various sites in the Netherlands that differed in their local PM emission sources. Our results are in support of other investigations showing that the chemical composition as well as oxidative potential are determinants of PM induced toxicity in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.