ArticleThe Biochemical journal2012
A global survey of CRM1-dependent nuclear export sequences in the human deubiquitinase family.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- A Deep-Learning Atlas of XPO1-Mediated Nuclear Export at Proteome Scale.bioRxiv : the preprint server for biology · 2026Article
- Mechanism of USP21 autoinhibition and histone H2AK119 deubiquitination.Science advances · 2025Article
- USP34 regulates endothelial PAR1 mRNA transcript expression and cellular signaling.Molecular biology of the cell · 2025Article
- circCD2AP promotes epithelial mesenchymal transition and stemness in bladder cancer by regulating FOXQ1/USP21 axis.iScience · 2024Article
- Insights Into the Properties, Biological Functions, and Regulation of USP21.Frontiers in pharmacology · 2022Review
- Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne regulates ubiquitin binding.The Journal of biological chemistry · 2020Article
- USP21 deubiquitinase promotes pancreas cancer cell stemness via Wnt pathway activation.Genes & development · 2019Article
- USP21 modulates Goosecoid function through deubiquitination.Bioscience reports · 2019Article
- Proteomic Analysis of Novel Components ofToxins · 2019Article
- Deubiquitinating enzyme USP3 controls CHK1 chromatin association and activation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- p38 inhibition provides anti-DNA virus immunity by regulation of USP21 phosphorylation and STING activation.The Journal of experimental medicine · 2017Article
- The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog.Nature communications · 2016Article
- Reply to Rodriguez: Mechanism of nuclear-cytosol shuttling of Usp12.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Description of USP12 nuclear export sequence.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Proteome-wide search for functional motifs altered in tumors: Prediction of nuclear export signals inactivated by cancer-related mutations.Scientific reports · 2016Article
- Identifying candidate genes for 2p15p16.1 microdeletion syndrome using clinical, genomic, and functional analysis.JCI insight · 2016Article
- ELM 2016--data update and new functionality of the eukaryotic linear motif resource.Nucleic acids research · 2016Article
- The business of deubiquitination - location, location, location.F1000Research · 2016Review
- LocNES: a computational tool for locating classical NESs in CRM1 cargo proteins.Bioinformatics (Oxford, England) · 2015Article
- NESmapper: accurate prediction of leucine-rich nuclear export signals using activity-based profiles.PLoS computational biology · 2014Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms that regulate the nucleocytoplasmic localization of human deubiquitinases remain largely unknown. The nuclear export receptor CRM1 binds to specific amino acid motifs termed NESs (nuclear export sequences). By using in silico prediction and experimental validation of candidate sequences, we identified 32 active NESs and 78 inactive NES-like motifs in human deubiquitinases. These results allowed us to evaluate the performance of three programs widely used for NES prediction, and to add novel information to the recently redefined NES consensus. The novel NESs identified in the present study reveal a subset of 22 deubiquitinases bearing motifs that might mediate their binding to CRM1. We tested the effect of the CRM1 inhibitor LMB (leptomycin B) on the localization of YFP (yellow fluorescent protein)- or GFP (green fluorescent protein)-tagged versions of six NES-bearing deubiquitinases [USP (ubiquitin-specific peptidase) 1, USP3, USP7, USP21, CYLD (cylindromatosis) and OTUD7B (OTU-domain-containing 7B)]. YFP-USP21 and, to a lesser extent, GFP-OTUD7B relocated from the cytoplasm to the nucleus in the presence of LMB, revealing their nucleocytoplasmic shuttling capability. Two sequence motifs in USP21 had been identified during our survey as active NESs in the export assay. Using site-directed mutagenesis, we show that one of these motifs mediates USP21 nuclear export, whereas the second motif is not functional in the context of full-length USP21.
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