ArticlePloS one2011
Efficient differentiation of embryonic stem cells into hepatic cells in vitro using a feeder-free basement membrane substratum.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- The Efficiency of Stem Cells (SCs) Differentiation into Functional Hepatocytes for Treating Liver Disorders: A Systematic Review.BioMed research international · 2023Pooled it
- Article
- Article
- Review
- The Hepatotoxicity of Alantolactone and Germacrone: Their Influence on Cholesterol and Lipid Metabolism in Differentiated HepaRG Cells.Nutrients · 2020Article
- Collagen vitrigel promotes hepatocytic differentiation of induced pluripotent stem cells into functional hepatocyte-like cells.Biology open · 2019Article
- Efficient Production of Hepatocyte-like Cells from Human-induced Pluripotent Stem Cells by Optimizing Growth Factors.International journal of organ transplantation medicine · 2018Article
- Acute effect of lactic acid on tumor-endothelial cell metabolic coupling in the tumor microenvironment.Oncology letters · 2016Article
- Extracellular matrix microarrays to study inductive signaling for endoderm specification.Acta biomaterialia · 2016Article
- Enhanced Ex Vivo Expansion of Human Hematopoietic Progenitors on Native and Spin Coated Acellular Matrices Prepared from Bone Marrow Stromal Cells.Stem cells international · 2016Article
- Hhex Is Necessary for the Hepatic Differentiation of Mouse ES Cells and Acts via Vegf Signaling.PloS one · 2016Article
- In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration.Protein & cell · 2015Review
- The study of energy metabolism in bladder cancer cells in co-culture conditions using a microfluidic chip.International journal of clinical and experimental medicine · 2015Article
- Extracellular Matrix and Integrins in Embryonic Stem Cell Differentiation.Biochemistry insights · 2015Review
- Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells.PloS one · 2015Article
- Stem Cell Strategies to Evaluate Idiosyncratic Drug-induced Liver Injury.Journal of clinical and translational hepatology · 2014Review
- An experimental approach to the generation of human embryonic stem cells equivalents.Molecular biotechnology · 2014Review
- Secreted cerberus1 as a marker for quantification of definitive endoderm differentiation of the pluripotent stem cells.PloS one · 2013Article
- Single-step protocol for the differentiation of human-induced pluripotent stem cells into hepatic progenitor-like cells.Biomedical reports · 2013Article
- Laminin α5 guides tissue patterning and organogenesis.Cell adhesion & migrationReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endoderm-inducing effect of the mesoderm-derived supportive cell line M15 on embryonic stem (ES) cells is partly mediated through the extracellular matrix, of which laminin α5 is a crucial component. Mouse ES or induced pluripotent stem cells cultured on a synthesized basement membrane (sBM) substratum, using an HEK293 cell line (rLN10-293 cell) stably expressing laminin-511, could differentiate into definitive endoderm and subsequently into pancreatic lineages. In this study, we investigated the differentiation on sBM of mouse and human ES cells into hepatic lineages. The results indicated that the BM components played an important role in supporting the regional-specific differentiation of ES cells into hepatic endoderm. We show here that knockdown of integrin β1 (Itgb1) in ES cells reduced their differentiation into hepatic lineages and that this is mediated through Akt signaling activation. Moreover, under optimal conditions, human ES cells differentiated to express mature hepatocyte markers and secreted high levels of albumin. This novel procedure for inducing hepatic differentiation will be useful for elucidating the molecular mechanisms controlling lineage-specific fates during gut regionalization. It could also represent an attractive approach to providing a surrogate cell source, not only for regenerative medicine, but also for pharmaceutical and toxicologic studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.