ArticlePloS one2011
Functional insight into the C-terminal extension of halolysin SptA from haloarchaeon Natrinema sp. J7.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A haloarchaeal VapBC toxin-antitoxin system: regulatory mechanism and role in establishing population heterogeneity to facilitate survival of subpopulations under stress conditions.Applied and environmental microbiology · 2026Article
- Identification and characterization of a novel halolysin from Halostella sp. PRR32 with two C-terminal extensions.Extremophiles : life under extreme conditions · 2025Article
- A TrmBL2-like transcription factor mediates the growth phase-dependent expression of halolysin SptA in a concentration-dependent manner inApplied and environmental microbiology · 2024Article
- Sec-Dependent Secretion of Subtilase SptE inApplied and environmental microbiology · 2022Article
- Mechanistic Insight into the Binding and Swelling Functions of Prepeptidase C-Terminal (PPC) Domains from Various Bacterial Proteases.Applied and environmental microbiology · 2019Article
- Halolysin SptA, a Serine Protease, Contributes to Growth-Phase Transition of HaloarchaeonFrontiers in microbiology · 2018Article
- Construction of Expression Shuttle Vectors for the HaloarchaeonArchaea (Vancouver, B.C.) · 2017Article
- Alternative Translation Initiation of a Haloarchaeal Serine Protease Transcript Containing Two In-Frame Start Codons.Journal of bacteriology · 2016Article
- Improved catalytic efficiency, thermophilicity, anti-salt and detergent tolerance of keratinase KerSMD by partially truncation of PPC domain.Scientific reports · 2016Article
- Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2.Archaea (Vancouver, B.C.) · 2015Article
- Chitin accelerates activation of a novel haloarchaeal serine protease that deproteinizes chitin-containing biomass.Applied and environmental microbiology · 2014Article
- Autocatalytic maturation of the Tat-dependent halophilic subtilase Nep produced by the archaeon Natrialba magadii.Journal of bacteriology · 2012Article
- Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension.Applied and environmental microbiology · 2012Article
- The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements.PloS one · 2012Article
- Diversity and subcellular distribution of archaeal secreted proteins.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Halolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish protease activity, which suggests that the CTE is not essential for enzyme folding. Mutational analysis suggests that residues Cys303 and Cys338 within the CTE form a disulfide bond that make this domain resistant to autocleavage and proteolysis under hypotonic conditions. Characterization of full-length and CTE-truncation enzymes indicates the CTE not only confers extra stability to the enzyme but also assists enzyme activity on protein substrates by facilitating binding at high salinities. Interestingly, homology modeling of the CTE yields a β-jelly roll-like structure similar to those seen in Claudin-binding domain of Clostridium perfringens enterotoxin (clostridial C-CPE) and collagen binding domain (CBD), and the CTE also possesses collagen-binding activity, making it a potential candidate as an anchoring unit in drug delivery systems.
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