ArticlePloS one2011
Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The Effect of Mice Adaptation Process on the Pathogenicity of Influenza A/South Africa/3626/2013 (H1N1)pdm09 Model Strain.International journal of molecular sciences · 2023Article
- Culture-Independent Workflow for Nanopore MinION-Based Sequencing of Influenza A Virus.Microbiology spectrum · 2023Article
- Influenza and antiviral resistance: an overview.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- A rapid pyrosequencing assay for the molecular detection of influenza viruses with reduced baloxavir susceptibility due to PA/I38X amino acid substitutions.Influenza and other respiratory viruses · 2020Article
- A Systematic Review Analyzing the Prevalence and Circulation of Influenza Viruses in Swine Population Worldwide.Pathogens (Basel, Switzerland) · 2020Review
- Laboratory Methods in Molecular Epidemiology: Viral Infections.Microbiology spectrum · 2018Review
- The use of pyrosequencing for detection of hemagglutinin mutations associated with increased pathogenicity of H5N1 avian influenza viruses in mammals.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Detection of adamantane-sensitive influenza A(H3N2) viruses in Australia, 2017: a cause for hope?Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
- A Pyrosequencing-Based Approach to High-Throughput Identification of Influenza A(H3N2) Virus Clades Harboring Antigenic Drift Variants.Journal of clinical microbiology · 2017Article
- Genotyping assay for differentiation of wild-type and vaccine viruses in subjects immunized with live attenuated influenza vaccine.PloS one · 2017Article
- Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray.SpringerPlus · 2016Article
- Inter-Seasonal Influenza is Characterized by Extended Virus Transmission and Persistence.PLoS pathogens · 2015Article
- Molecular diagnosis of respiratory viruses.Pathology · 2015Review
- Deep sequencing: becoming a critical tool in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2014Review
- Evaluation of Alere i Influenza A&B for rapid detection of influenza viruses A and B.Journal of clinical microbiology · 2014Article
- Clinical PathoScope: rapid alignment and filtration for accurate pathogen identification in clinical samples using unassembled sequencing data.BMC bioinformatics · 2014Article
- Incorporation of a proteotyping approach using mass spectrometry for surveillance of influenza virus in cell-cultured strains.Journal of clinical microbiology · 2014Article
- Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2.Nanoscale research letters · 2014Article
- Article
- Rapid strategy for screening by pyrosequencing of influenza virus reassortants--candidates for live attenuated vaccines.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiven the continuing co-circulation of the 2009 H1N1 pandemic influenza A viruses with seasonal H3N2 viruses, rapid and reliable detection of newly emerging influenza reassortant viruses is important to enhance our influenza surveillance. METHODOLOGY/PRINCIPAL
findingsA novel pyrosequencing assay was developed for the rapid identification and subtyping of potential human influenza A virus reassortants based on all eight gene segments of the virus. Except for HA and NA genes, one universal set of primers was used to amplify and subtype each of the six internal genes. With this method, all eight gene segments of 57 laboratory isolates and 17 original specimens of seasonal H1N1, H3N2 and 2009 H1N1 pandemic viruses were correctly matched with their corresponding subtypes. In addition, this method was shown to be capable of detecting reassortant viruses by correctly identifying the source of all 8 gene segments from three vaccine production reassortant viruses and three H1N2 viruses. CONCLUSIONS/SIGNIFICANCE: In summary, this pyrosequencing assay is a sensitive and specific procedure for screening large numbers of viruses for reassortment events amongst the commonly circulating human influenza A viruses, which is more rapid and cheaper than using conventional sequencing approaches.
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