ArticleBiochemical pharmacology2011
Ethanol impairs muscarinic receptor-induced neuritogenesis in rat hippocampal slices: Role of astrocytes and extracellular matrix proteins.
Article in Biochemical pharmacology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Synaptogenesis by Cholinergic Stimulation of Astrocytes.Neurochemical research · 2023Article
- The role of PPAR-γ in memory deficits induced by prenatal and lactation alcohol exposure in mice.Molecular psychiatry · 2023Article
- Synaptogenesis by Cholinergic Stimulation of Astrocytes.Research square · 2023Article
- Assessment of ethanol exposure from hand sanitizer use and potential for developmental toxicity in nursing infants.Journal of applied toxicology : JAT · 2022Review
- Microglia and astrocytes show limited, acute alterations in morphology and protein expression following a single developmental alcohol exposure.Journal of neuroscience research · 2021Article
- Neonatal Ethanol and Choline Treatments Alter the Morphology of Developing Rat Hippocampal Pyramidal Neurons in Opposite Directions.Neuroscience · 2018Article
- Stable Histone Methylation Changes at Proteoglycan Network Genes Following Ethanol Exposure.Frontiers in genetics · 2018Article
- What the Spectrum of Microglial Functions Can Teach us About Fetal Alcohol Spectrum Disorder.Frontiers in synaptic neuroscience · 2017Review
- Effects of Ethanol on Brain Extracellular Matrix: Implications for Alcohol Use Disorder.Alcoholism, clinical and experimental research · 2016Review
- Developmental alcohol exposure leads to a persistent change on astrocyte secretome.Journal of neurochemistry · 2016Article
- Ethanol deregulates Mecp2/MeCP2 in differentiating neural stem cells via interplay between 5-methylcytosine and 5-hydroxymethylcytosine at the Mecp2 regulatory elements.Experimental neurology · 2015Article
- Fetal Alcohol Spectrum Disorders: An Overview from the Glia Perspective.Frontiers in integrative neuroscience · 2015Review
- Diazinon and diazoxon impair the ability of astrocytes to foster neurite outgrowth in primary hippocampal neurons.Toxicology and applied pharmacology · 2014Article
- Glia and neurodevelopment: focus on fetal alcohol spectrum disorders.Frontiers in pediatrics · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
In an in vitro co-culture system of astrocytes and neurons, stimulation of cholinergic muscarinic receptors in astrocytes had been shown to cause neuritogenesis in hippocampal neurons, and this effect was inhibited by ethanol. The present study sought to confirm these earlier findings in a more complex system, in vitro rat hippocampal slices in culture. Exposure of hippocampal slices to the cholinergic agonist carbachol (1mM for 24h) induced neurite outgrowth in hippocampal pyramidal neurons, which was mediated by activation of muscarinic M3 receptors. Specifically, carbachol induced a >4-fold increase in the length of the longest neurite, and a 4-fold increase in the length of minor neurites and in the number of branches. Co-incubation of carbachol with ethanol (50mM) resulted in significant inhibition of the effects induced by carbachol on all parameters measured. Neurite outgrowth in CNS neurons is dependent on various permissive factors that are produced and released by glial cells. In hippocampal slices carbachol increased the levels of two extracellular matrix protein, fibronectin and laminin-1, by 1.6-fold, as measured by Western blot. Co-incubation of carbachol with ethanol significantly inhibited these increases. Carbachol-induced increases in levels of extracellular matrix proteins were antagonized by a M3 muscarinic receptor antagonist. Furthermore, function-blocking fibronectin or laminin-1 antibodies antagonized the effect of carbachol on neurite outgrowth. These results indicate that in hippocampal slices stimulation of muscarinic M3 receptors induces neurite outgrowth, which is mediated by fibronectin and laminin-1, two extracellular matrix proteins released by astrocytes. By decreasing fibronectin and laminin levels ethanol prevents carbachol-induced neuritogenesis. These findings highlight the importance of glial-neuronal interactions as important targets in the developmental neurotoxicity of alcohol.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.