ReviewMicrobial biotechnology2012
Strategies for discovery and improvement of enzyme function: state of the art and opportunities.
Review in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Discovery of a multifunctional chitinase-cellulase from Thermococcus chitonophagus with expanded polysaccharide specificity.Biotechnology for biofuels and bioproducts · 2026Article
- Enzymatic Synthesis of β-lactam Antibiotics: From Enzyme Dataset Construction to Action Mechanism and Semi-Rational Design.Current protein & peptide science · 2026Review
- Discovery and engineering of bifunctional enzymes for lignocellulose degradation: Metagenomic and computational approaches.Biotechnology reports (Amsterdam, Netherlands) · 2025Review
- Advancing Enzyme-Based Detoxification Prediction with ToxZyme: An Ensemble Machine Learning Approach.Toxins · 2025Article
- A novel salt- and organic solvent-tolerant phosphite dehydrogenase fromFrontiers in bioengineering and biotechnology · 2023Article
- Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Insufficiency in functional genomics studies, data, and applications: A case study of bio-prospecting research in ruminant microbiome.Frontiers in genetics · 2022Review
- Discovery and Structural Analysis to Improve the Enantioselectivity of Hydroxynitrile Lyase fromACS omega · 2020Article
- Predicting metabolic pathways of plant enzymes without using sequence similarity: Models from machine learning.The plant genome · 2020Article
- Understanding High-Salt and Cold Adaptation of a Polyextremophilic Enzyme.Microorganisms · 2020Article
- Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives.Molecular biotechnology · 2019Review
- nanoDSF as screening tool for enzyme libraries and biotechnology development.The FEBS journal · 2019Article
- Long-term protein packaging in bio-ionic liquids: Improved catalytic activity and enhanced stability of cytochrome C against multiple stresses.Green chemistry : an international journal and green chemistry resource : GC · 2017Article
- Mining of Microbial Genomes for the Novel Sources of Nitrilases.BioMed research international · 2017Article
- Microbial enzymes: tools for biotechnological processes.Biomolecules · 2014Review
- Function and biotechnology of extremophilic enzymes in low water activity.Aquatic biosystems · 2012Article
- Putting bugs to the blush: metabolic engineering for phenylpropanoid-derived products in microorganisms.BioengineeredArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developments in biocatalysis have been largely fuelled by consumer demands for new products, industrial attempts to improving existing process and minimizing waste, coupled with governmental measures to regulate consumer safety along with scientific advancements. One of the major hurdles to application of biocatalysis to chemical synthesis is unavailability of the desired enzyme to catalyse the reaction to allow for a viable process development. Even when the desired enzyme is available it often forces the process engineers to alter process parameters due to inadequacies of the enzyme, such as instability, inhibition, low yield or selectivity, etc. Developments in the field of enzyme or reaction engineering have allowed access to means to achieve the ends, such as directed evolution, de novo protein design, use of non-conventional media, using new substrates for old enzymes, active-site imprinting, altering temperature, etc. Utilization of enzyme discovery and improvement tools therefore provides a feasible means to overcome this problem. Judicious employment of these tools has resulted in significant advancements that have leveraged the research from laboratory to market thus impacting economic growth; however, there are further opportunities that have not yet been explored. The present review attempts to highlight some of these achievements and potential opportunities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.