ArticleTraffic (Copenhagen, Denmark)2011
Hotspots organize clathrin-mediated endocytosis by efficient recruitment and retention of nucleating resources.
Article in Traffic (Copenhagen, Denmark), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed.
- Reframing clathrin-mediated viral entry: evidence standards, context dependence and host-directed vulnerabilities.Frontiers in cellular and infection microbiology · 2026Review
- Cargo-selective regulation of clathrin-mediated endocytosis by AMP-activated protein kinase.iScience · 2025Article
- Circular RNAs as Biomarkers in the Diagnosis of Human Diseases.Advances in experimental medicine and biology · 2025Review
- Dynamin1 long- and short-tail isoforms exploit distinct recruitment and spatial patterns to form endocytic nanoclusters.Nature communications · 2024Article
- Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion.Cellular and molecular life sciences : CMLS · 2024Article
- Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling.The Journal of cell biology · 2022Article
- Endocytosis and the internalization of pathogenic organisms: focus on phosphoinositides.F1000Research · 2020Review
- Regulation of Clathrin-Mediated Endocytosis.Annual review of biochemistry · 2018Review
- Mechanisms of clathrin-mediated endocytosis.Nature reviews. Molecular cell biology · 2018Review
- Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis.PLoS biology · 2018Article
- Loss of PTEN promotes formation of signaling-capable clathrin-coated pits.Journal of cell science · 2018Article
- A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells.PLoS biology · 2018Article
- Selective regulation of clathrin-mediated epidermal growth factor receptor signaling and endocytosis by phospholipase C and calcium.Molecular biology of the cell · 2017Article
- Endocytosis, Metastasis and Beyond: Multiple Facets of SNX9.Trends in cell biology · 2017Review
- Cell spreading area regulates clathrin-coated pit dynamics on micropatterned substrate.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Article
- Endocytic accessory factors and regulation of clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Imaging and modeling the dynamics of clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Enhanced receptor-clathrin interactions induced by N-glycan-mediated membrane micropatterning.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Systematic spatial mapping of proteins at exocytic and endocytic structures.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The formation of clathrin-coated pits (CCPs) at the plasma membrane has been reported to sometimes occur repeatedly at predefined sites. However, defining such CCP 'hotspots' structurally and mechanistically has been difficult due to the dynamic and heterogeneous nature of CCPs. Here, we explore the molecular requirements for hotspots using a global assay of CCP dynamics. Our data confirmed that a subset of CCPs is nucleated at spatially distinct sites. The degree of clustering of nucleation events at these sites is dependent on the integrity of cortical actin, and the availability of certain resources, including the adaptor protein AP-2 and the phospholipid PI(4,5)P(2) . We observe that modulation in the expression level of FCHo1 and 2, which have been reported to initiate CCPs, affects only the number of nucleations. Modulation in the expression levels of other accessory proteins, such as SNX9, affects the spatial clustering of CCPs but not the number of nucleations. On the basis of these findings, we distinguish two classes of accessory proteins in clathrin-mediated endocytosis (CME): nucleation factors and nucleation organizers. Finally, we observe that clustering of transferrin receptors spatially randomizes pit nucleation and thus reduces the role of hotspots. On the basis of these data, we propose that hotspots are specialized cortical actin patches that organize CCP nucleations from within the cell by more efficient recruitment and/or retention of the resources required for CCP nucleation partially due to the action of nucleation organizers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.