ArticleCurrent microbiology2011
Prevalence of acanthamoeba from tap water in rio grande do Sul, Brazil.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Molecular identification and genotype distribution of Acanthamoeba in environmental water.Irish journal of medical science · 2026Article
- Identification of Acantamoeba isolated from water resources located in the northwest of Iran by using the high-resolution melting analysis assay.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Molecular identification of potentially pathogenic free-living amoebae in environmental samples from urban areas of Kerman, Southeastern Iran.Parasite epidemiology and control · 2026Article
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
- Isolation, identification of pathogenicJournal of advanced veterinary and animal research · 2018Article
- Article
- Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers.Memorias do Instituto Oswaldo Cruz · 2017Article
- Free-living amoebae in the water resources of Iran: a systematic review.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Review
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Isolation ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Evaluation of the activity of new cationic carbosilane dendrimers on trophozoites and cysts of Acanthamoeba polyphaga.Parasitology research · 2015Article
- Molecular Characterization of Pathogenic Acanthamoeba Isolated from Drinking and Recreational water in East Azerbaijan, Northwest Iran.Environmental health insights · 2015Article
- Detection and Molecular Characterization of Potentially Pathogenic Free-living Amoebae from Water Sources in Kish Island, Southern Iran.Microbiology insights · 2015Article
- Characterization of isolates of Acanthamoeba from the nasal mucosa and cutaneous lesions of dogs.Current microbiology · 2014Article
- In vitro evaluation of the effectiveness of new water-stable cationic carbosilane dendrimers against Acanthamoeba castellanii UAH-T17c3 trophozoites.Parasitology research · 2013Article
- Isolation and identification of free-living amoebae from tap water in Sivas, Turkey.BioMed research international · 2013Article
- Prevalence of Acanthamoeba spp. (Sarcomastigophora: Acanthamoebidae) in wild populations of Aedes aegypti (Diptera: Culicidae).Parasitology research · 2012Article
- Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.Iranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 136 samples of tap water were collected from state and municipal schools between March and November 2009. The samples were filtered through cellulose nitrate membranes that were seeded at non-nutrient agar 1.5% containing an overlayer of Escherichia coli suspension. Thirty-one (22.79%) tap water samples investigated were found positive for free-living amoebae (FLA). From these, 13 presented as FLA that seems to belong to the genus Acanthamoeba. All samples of FLA were cloned and identified as belonging to the genus Acanthamoeba by the morphology of cysts and trophozoites and by PCR using genus-specific primers that amplify the ASA.S1 region of 18S rDNA gene. Physiological tests of thermotolerance and osmotolerance were used to evaluate the pathogenicity of the isolates. The sequencing analysis by comparing the sequences submitted to GenBank, showed genotype distribution into groups T2, T2/T6, T6, and T4. In tests of thermotolerance and osmotolerance, 50% of the isolates had a low pathogenic potential. The results indicated the presence of Acanthamoeba in tap water in Rio Grande do Sul, Brazil, revealing its importance and the need for more epidemiological studies to determine their distribution in the environment and its pathogenic potential.
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