Evidence map›Paper›PMID 21880866›Full record

ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2011

Local delivery of a PKCε-activating peptide limits ischemia reperfusion injury in the aged female rat heart.

T S Lancaster, S J Jefferson, D H Korzick

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Estrogen and the female heart.Molecular and cellular endocrinology · 2014
    Review
  11. Chronic alcohol consumption disrupts myocardial protein balance and function in aged, but not adult, female F344 rats.American journal of physiology. Regulatory, integrative and comparative physiology · 2014
    Article
  12. Article
  13. Promoter methylation of Egr-1 site contributes to fetal hypoxia-mediated PKCε gene repression in the developing heart.American journal of physiology. Regulatory, integrative and comparative physiology · 2013
    Article
  14. Review
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

T S LancasterDepartment of Kinesiology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
S J Jefferson
D H Korzick
Pennsylvania State University · US

Funding

Mitochondrial and Nuclear Mechanisms of Estrogen Receptor-Mediated CardioprotectiR01HL091097 · NHLBI · PENNSYLVANIA STATE UNIVERSITY, THE · PI KORZICK, DONNA HOPE · 2009 to 2012
$1.7M
Novel Mitochondrial Mechanisms of Ethanol-Induced Cardiac Injury in Aged FemalesRC2AA019403 · NIAAA · PENNSYLVANIA STATE UNIVERSITY, THE · PI KORZICK, DONNA HOPE · 2009 to 2010
$1.0M
NHLBI NIH HHS R01 HL091097NHLBI NIH HHS R01-HL-091097-01A2S1NIAAA NIH HHS RC2 AA019403
6 · The paper itself

Abstract

Reduced efficacy of cardioprotective interventions in the aged female heart, including estrogen replacement, highlights the need for alternative therapeutics to reduce myocardial ischemia-reperfusion (I/R) injury in postmenopausal women. Here, we sought to determine the efficacy of protein kinase-Cε (PKCε)-mediated cardioprotection in the aged, estradiol-deficient rat heart. Infarct size and functional recovery were assessed in Langendorff-perfused hearts from adult (5 mo) or aged (23 mo) female Fisher 344 ovary-intact or ovariectomized (OVX) rats administered a PKCε-activator, receptor for activated C kinase (ψεRACK) prior to 47-min ischemia and 60-min reperfusion. Proteomic analysis was conducted on left ventricular mitochondrial fractions treated with ψεRACK prior to I/R, utilizing isobaric tags for relative and absolute quantitation (iTRAQ) 8plex labeling and tandem mass spectrometry. Real-time PCR was utilized to assess connexin 43 (Cx43) and RACK2 mRNA post-I/R. Greater infarct size in aged OVX (78%) vs. adult (37%) was reduced by ψεRACK (35%, P < 0.0001) and associated with greater mitochondrial PKCε localization (P < 0.0003). Proteomic analysis revealed three novel mitochondrial targets of PKCε-mediated cardioprotection with aging (P < 0.05): the antioxidant enzymes glutathione peroxidase (GPX) and MnSOD2, and heat shock protein 10. Finally, decreased levels of Cx43 and RACK2 mRNA seen with age were partially abrogated by administration of ψεRACK (P < 0.05). The mechanisms described here may represent important therapeutic candidates for the treatment of acute myocardial infarction in postmenopausal women and age-associated estradiol deficiency.

Indexed as

AgingAge FactorsAnimalsCardiotonic AgentsChaperonin 10Connexin 43Disease Models, AnimalEnzyme ActivationEstradiolFemaleGlutathione PeroxidaseMembrane ProteinsMitochondria, HeartMyocardial InfarctionMyocardial Reperfusion InjuryMyocardiumCardiotonic AgentsChaperonin 10Connexin 43Copb1 protein, ratEstradiolGlutathione PeroxidaseHis-Asp-Ala-Pro-Ile-Gly-Tyr-AspMembrane ProteinsOligopeptidesPrkce protein, ratProtein Kinase C-epsilonProto-Oncogene Proteins c-aktRNA, MessengerSuperoxide DismutaseSuperoxide Dismutase 2

Identifiers

PMID21880866
PMCPMC3213936
OpenAlexW2104172033

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.