ArticleJournal of virology2011
The encapsidated genome of Microplitis demolitor bracovirus integrates into the host Pseudoplusia includens.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Systematic profiling of nudivirus-like genes reveals conserved and differentiated roles in a domesticated endogenous virus.PLoS pathogens · 2026Article
- Exploring the Determinants of Polydnavirus Chromosomal Integration Across Host-Parasitoid Wasp Systems.Genome biology and evolution · 2026Article
- Horizontal transfers of polydnavirus segments extend the known range of parasitoid attacks to stick insects and orthopterans.Molecular biology and evolution · 2026Article
- The role of a viral symbiont in the thermal mismatch of host-parasitoid interactions.The Journal of experimental biology · 2026Article
- Functional characterization ofJournal of virology · 2023Article
- Massive Somatic and Germline Chromosomal Integrations of Polydnaviruses in Lepidopterans.Molecular biology and evolution · 2023Article
- Genome, host genome integration, and gene expression inFrontiers in microbiology · 2023Article
- The Complete Genome ofJournal of virology · 2022Article
- Bracovirus Sneaks Into Apoptotic Bodies Transmitting Immunosuppressive Signaling Driven by Integration-Mediated eIF5A Hypusination.Frontiers in immunology · 2022Article
- Genome-Wide Patterns of Bracovirus Chromosomal Integration into Multiple Host Tissues during Parasitism.Journal of virology · 2021Article
- Bracoviruses recruit host integrases for their integration into caterpillar's genome.PLoS genetics · 2021Article
- Article
- Genome-Wide Profiling ofFrontiers in microbiology · 2020Article
- Cotesia congregata Bracovirus Circles EncodingJournal of virology · 2018Article
- Review
- Article
- Microplitis demolitor Bracovirus Proviral Loci and Clustered Replication Genes Exhibit Distinct DNA Amplification Patterns during Replication.Journal of virology · 2015Article
- Recurrent Domestication by Lepidoptera of Genes from Their Parasites Mediated by Bracoviruses.PLoS genetics · 2015Article
- Genomic and Proteomic Analyses Indicate that Banchine and Campoplegine Polydnaviruses Have Similar, if Not Identical, Viral Ancestors.Journal of virology · 2015Article
- Polydnaviruses: From discovery to current insights.Virology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polydnaviruses (PDVs) are symbionts of parasitoid wasps that function as gene delivery vehicles in the insects (hosts) that the wasps parasitize. PDVs persist in wasps as integrated proviruses but are packaged as circularized and segmented double-stranded DNAs into the virions that wasps inject into hosts. In contrast, little is known about how PDV genomic DNAs persist in host cells. Microplitis demolitor carries Microplitis demolitor bracovirus (MdBV) and parasitizes the host Pseudoplusia includens. MdBV infects primarily host hemocytes and also infects a hemocyte-derived cell line from P. includens called CiE1 cells. Here we report that all 15 genomic segments of the MdBV encapsidated genome exhibited long-term persistence in CiE1 cells. Most MdBV genes expressed in hemocytes were persistently expressed in CiE1 cells, including members of the glc gene family whose products transformed CiE1 cells into a suspension culture. PCR-based integration assays combined with cloning and sequencing of host-virus junctions confirmed that genomic segments J and C persisted in CiE1 cells by integration. These genomic DNAs also rapidly integrated into parasitized P. includens. Sequence analysis of wasp-viral junction clones showed that the integration of proviral segments in M. demolitor was associated with a wasp excision/integration motif (WIM) known from other bracoviruses. However, integration into host cells occurred in association with a previously unknown domain that we named the host integration motif (HIM). The presence of HIMs in most MdBV genomic DNAs suggests that the integration of each genomic segment into host cells occurs through a shared mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.