ArticleThe Journal of biological chemistry2011
Local cytosolic Ca2+ elevations are required for stromal interaction molecule 1 (STIM1) de-oligomerization and termination of store-operated Ca2+ entry.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Two-pore channel-2 controls calmodulin-dependent STIM1 inactivation.Nature communications · 2026Article
- T cell cholesterol transport links intestinal immune responses to dietary lipid absorption.Science (New York, N.Y.) · 2025Article
- Paraquat is an agonist of STIM1 and increases intracellular calcium levels.Communications biology · 2022Article
- Alteration of STIM1/Orai1-Mediated SOCE in Skeletal Muscle: Impact in Genetic Muscle Diseases and Beyond.Cells · 2021Review
- Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling.Nature communications · 2020Article
- Tmem178 negatively regulates store-operated calcium entry in myeloid cells via association with STIM1.Journal of autoimmunity · 2019Article
- ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain.The Journal of physiology · 2019Article
- Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers.Nature communications · 2017Article
- Characterization of the ER-Targeted Low Affinity Ca(2+) Probe D4ER.Sensors (Basel, Switzerland) · 2016Article
- Diseases caused by mutations in ORAI1 and STIM1.Annals of the New York Academy of Sciences · 2015Review
- Store-Operated Calcium Channels.Physiological reviews · 2015Review
- A STIM2 splice variant negatively regulates store-operated calcium entry.Nature communications · 2015Article
- Structural and functional mechanisms of CRAC channel regulation.Journal of molecular biology · 2015Review
- Redox regulation of store-operated Ca2+ entry.Antioxidants & redox signaling · 2014Review
- IP3-mediated STIM1 oligomerization requires intact mitochondrial Ca2+ uptake.Journal of cell science · 2014Article
- Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA.Nature communications · 2014Article
- Reversal of murine epidermal atrophy by topical modulation of calcium signaling.The Journal of investigative dermatology · 2014Article
- Molecular regulation of CRAC channels and their role in lymphocyte function.Cellular and molecular life sciences : CMLS · 2013Review
- Activation of store-operated calcium entry in airway smooth muscle cells: insight from a mathematical model.PloS one · 2013Article
- Orai-STIM-mediated Ca2+ release from secretory granules revealed by a targeted Ca2+ and pH probe.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ca(2+) depletion of the endoplasmic reticulum (ER) activates the ubiquitous store-operated Ca(2+) entry (SOCE) pathway that sustains long-term Ca(2+) signals critical for cellular functions. ER Ca(2+) depletion initiates the oligomerization of stromal interaction molecules (STIM) that control SOCE activation, but whether ER Ca(2+) refilling controls STIM de-oligomerization and SOCE termination is not known. Here, we correlate the changes in free luminal ER Ca(2+) concentrations ([Ca(2+)](ER)) and in STIM1 oligomerization, using fluorescence resonance energy transfer (FRET) between CFP-STIM1 and YFP-STIM1. We observed that STIM1 de-oligomerized at much lower [Ca(2+)](ER) levels during store refilling than it oligomerized during store depletion. We then refilled ER stores without adding exogenous Ca(2+) using a membrane-permeable Ca(2+) chelator to provide a large reservoir of buffered Ca(2+). This procedure rapidly restored pre-stimulatory [Ca(2+)](ER) levels but did not trigger STIM1 de-oligomerization, the FRET signals remaining elevated as long as the external [Ca(2+)] remained low. STIM1 dissociation evoked by Ca(2+) readmission was prevented by SOC channel inhibition and was associated with cytosolic Ca(2+) elevations restricted to STIM1 puncta, indicating that Ca(2+) acts on a cytosolic target close to STIM1 clusters. These data indicate that the refilling of ER Ca(2+) stores is not sufficient to induce STIM1 de-oligomerization and that localized Ca(2+) elevations in the vicinity of assembled SOCE complexes are required for the termination of SOCE.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.