ArticleThe Journal of biological chemistry2011
Adaptor protein-3 (AP-3) complex mediates the biogenesis of acidocalcisomes and is essential for growth and virulence of Trypanosoma brucei.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- Polyphosphate is a novel pro-inflammatory regulator of mast cells and is located in acidocalcisomes.The Journal of biological chemistry · 2012Trial
- Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes.Microbiology and molecular biology reviews : MMBR · 2024Review
- Zn deficiency disrupts Cu and S homeostasis in Chlamydomonas resulting in over accumulation of Cu and Cysteine.Metallomics : integrated biometal science · 2023Article
- An X-Domain Phosphoinositide Phospholipase C (PI-PLC-like) ofPathogens (Basel, Switzerland) · 2023Article
- New insights into the role of acidocalcisomes in trypanosomatids.The Journal of eukaryotic microbiology · 2022Review
- TbVps41 regulates trafficking of endocytic but not biosynthetic cargo to lysosomes of bloodstream forms of Trypanosoma brucei.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Transcriptional remodeling during metacyclogenesis inVirulence · 2020Article
- Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae.Frontiers in plant science · 2020Review
- Importance of polyphosphate in theMicrobial cell (Graz, Austria) · 2018Article
- Magnesium uptake by connecting fluid-phase endocytosis to an intracellular inorganic cation filter.Nature communications · 2017Article
- Acidocalcisomes of eukaryotes.Current opinion in cell biology · 2016Review
- Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri.Scientific reports · 2016Article
- Polyphosphate and acidocalcisomes.Biochemical Society transactions · 2016Review
- Vacuolar ATPase depletion affects mitochondrial ATPase function, kinetoplast dependency, and drug sensitivity in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Rab32 is essential for maintaining functional acidocalcisomes, and for growth and infectivity of Trypanosoma cruzi.Journal of cell science · 2015Article
- The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp.Cell calcium · 2015Review
- Proteomic analysis of the acidocalcisome, an organelle conserved from bacteria to human cells.PLoS pathogens · 2014Article
- Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas.Nature chemical biology · 2014Article
- Lysosome-related organelles as mediators of metal homeostasis.The Journal of biological chemistry · 2014Review
- Acidocalcisome is required for autophagy in Trypanosoma brucei.Autophagy · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Acidocalcisomes are acidic calcium and polyphosphate storage organelles found in a diverse range of organisms. Here we present evidence that the biogenesis of acidocalcisomes in Trypanosoma brucei is linked to the expression of adaptor protein-3 (AP-3) complex. Localization studies in cell lines expressing β3 and δ subunits of AP-3 fused to epitope tags revealed their partial co-localization with the vacuolar proton pyrophosphatase, a marker of acidocalcisomes, with the Golgi marker Golgi reassembly and stacking protein, and with antibodies against the small GTPase Rab11. Ablation of the β3 subunit by RNA interference (RNAi) resulted in disappearance of acidocalcisomes from both procyclic and bloodstream form trypanosomes, as revealed by immmunofluorescence and electron microscopy assays, with no alterations in trafficking of different markers to lysosomes. Knockdown of the β3 subunit resulted in lower acidic calcium, pyrophosphate, and polyphosphate content as well as defects in growth in culture, resistance to osmotic stress, and virulence in mice. Similar results were obtained by knocking down the expression of the δ subunit of AP-3. These results indicate that AP-3 is essential for the biogenesis of acidocalcisomes and for growth and virulence of T. brucei.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.