ArticleBMC genomics2011
A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Quorum-sensing regulator LsrR modulates resistance to oxidative stress by interfering with sulfate assimilation in avian pathogenicJournal of bacteriology · 2026Article
- Strain-specific strategies underlie convergent phosphate solubilization inISME communications · 2025Article
- Ser/Thr protein kinase Stk1 phosphorylates the key transcriptional regulator AlgR to modulate virulence and resistance inVirulence · 2024Article
- Model-driven characterization of functional diversity ofMicrobial genomics · 2024Article
- BosR: A novel biofilm-specific regulator inFrontiers in microbiology · 2022Article
- Evaluation of small molecule kinase inhibitors as novel antimicrobial and antibiofilm agents.Chemical biology & drug design · 2021Review
- Beyond dueling: roles of the type VI secretion system in microbiome modulation, pathogenesis and stress resistance.Stress biology · 2021Review
- Pseudomonas aeruginosa transcriptome adaptations from colonization to biofilm infection of skin wounds.Scientific reports · 2021Article
- Oxidative Stress Response inPathogens (Basel, Switzerland) · 2021Review
- PmtA Regulates Pyocyanin Expression and Biofilm Formation inFrontiers in microbiology · 2021Article
- Impacts of Ser/Thr Protein Kinase Stk1 on the Proteome, Twitching Motility, and Competitive Advantage inFrontiers in microbiology · 2021Article
- Type VI Secretion Systems Present New Insights on PathogenicFrontiers in cellular and infection microbiology · 2018Review
- Transcriptional Responses of the Bacterium Burkholderia terrae BS001 to the Fungal Host Lyophyllum sp. Strain Karsten under Soil-Mimicking Conditions.Microbial ecology · 2017Article
- Novel drug targets in cell wall biosynthesis exploited by gene disruption in Pseudomonas aeruginosa.PloS one · 2017Article
- The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation inFrontiers in microbiology · 2017Article
- Wavelength-normalized spectroscopic analysis ofBiomedical optics express · 2016Article
- Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp. avenae by Principle Component Analysis.International journal of molecular sciences · 2015Article
- Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression.Journal of microbiology (Seoul, Korea) · 2015Article
- Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique.Frontiers in microbiology · 2015Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genome of Pseudomonas aeruginosa contains at least three genes encoding eukaryotic-type Ser/Thr protein kinases, one of which, ppkA, has been implicated in P. aeruginosa virulence. Together with the adjacent pppA phosphatase gene, they belong to the type VI secretion system (H1-T6SS) locus, which is important for bacterial pathogenesis. To determine the biological function of this protein pair, we prepared a pppA-ppkA double mutant and characterised its phenotype and transcriptomic profiles.
resultsPhenotypic studies revealed that the mutant grew slower than the wild-type strain in minimal media and exhibited reduced secretion of pyoverdine. In addition, the mutant had altered sensitivity to oxidative and hyperosmotic stress conditions. Consequently, mutant cells had an impaired ability to survive in murine macrophages and an attenuated virulence in the plant model of infection. Whole-genome transcriptome analysis revealed that pppA-ppkA deletion affects the expression of oxidative stress-responsive genes, stationary phase σ-factor RpoS-regulated genes, and quorum-sensing regulons. The transcriptome of the pppA-ppkA mutant was also analysed under conditions of oxidative stress and showed an impaired response to the stress, manifested by a weaker induction of stress adaptation genes as well as the genes of the SOS regulon. In addition, expression of either RpoS-regulated genes or quorum-sensing-dependent genes was also affected. Complementation analysis confirmed that the transcription levels of the differentially expressed genes were specifically restored when the pppA and ppkA genes were expressed ectopically.
conclusionsOur results suggest that in addition to its crucial role in controlling the activity of P. aeruginosa H1-T6SS at the post-translational level, the PppA-PpkA pair also affects the transcription of stress-responsive genes. Based on these data, it is likely that the reduced virulence of the mutant strain results from an impaired ability to survive in the host due to the limited response to stress conditions.
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