ArticleThe Journal of biological chemistry2011
Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
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- Structure-Function Diversity of Calcium-Binding Proteins (CaBPs): Key Roles in Cell Signalling and Disease.Cells · 2025Review
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- VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.The EMBO journal · 2019Article
- Calcium Sensors in Neuronal Function and Dysfunction.Cold Spring Harbor perspectives in biology · 2019Review
- Caldendrin and Calneurons-EF-Hand CaM-Like Calcium Sensors With Unique Features and Specialized Neuronal Functions.Frontiers in molecular neuroscience · 2019Review
- Neuronal Calcium and cAMP Cross-Talk Mediated by Cannabinoid CBFrontiers in cell and developmental biology · 2018Article
- Calneuron 1 Increased CaHypertension (Dallas, Tex. : 1979) · 2018Article
- Cocaine-Induced Chromatin Modifications Associate With Increased Expression and Three-Dimensional Looping of Auts2.Biological psychiatry · 2017Article
- Alternative splicing, expression and cellular localization of Calneuron-1 in the rat and human brain.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
- Sense and specificity in neuronal calcium signalling.Biochimica et biophysica acta · 2015Review
- Intracellular calcium levels determine differential modulation of allosteric interactions within G protein-coupled receptor heteromers.Chemistry & biology · 2014Article
- Analysis of Y-P30/Dermcidin expression and properties of the Y-P30 peptide.BMC research notes · 2014Article
- Solution NMR structure of the Ca2+-bound N-terminal domain of CaBP7: a regulator of golgi trafficking.The Journal of biological chemistry · 2012Article
- Evolution and functional diversity of the Calcium Binding Proteins (CaBPs).Frontiers in molecular neuroscience · 2012Article
- Ca(2+) sensor proteins in dendritic spines: a race for Ca(2+).Frontiers in molecular neuroscience · 2012Article
- NCS-1 associates with adenosine A(2A) receptors and modulates receptor function.Frontiers in molecular neuroscience · 2012Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calneuron-1 and -2 are neuronal EF-hand-type calcium sensor proteins that are prominently targeted to trans-Golgi network membranes and impose a calcium threshold at the Golgi for phosphatidylinositol 4-OH kinase IIIβ activation and the regulated local synthesis of phospholipids that are crucial for TGN-to-plasma membrane trafficking. In this study, we show that calneurons are nonclassical type II tail-anchored proteins that are post-translationally inserted into the endoplasmic reticulum membrane via an association of a 23-amino acid-long transmembrane domain (TMD) with the TRC40/Asna1 chaperone complex. Following trafficking to the Golgi, calneurons are probably retained in the TGN because of the length of the TMD and phosphatidylinositol 4-phosphate lipid binding. Both calneurons rapidly self-associate in vitro and in vivo via their TMD and EF-hand containing the N terminus. Although dimerization and potentially multimerization precludes TRC40/Asna1 binding and thereby membrane insertion, we found no evidence for a cytosolic pool of calneurons and could demonstrate that self-association of calneurons is restricted to membrane-inserted protein. The dimerization properties and the fact that they, unlike every other EF-hand calmodulin-like Ca(2+) sensor, are always associated with membranes of the secretory pathway, including vesicles and plasma membrane, suggests a high degree of spatial segregation for physiological target interactions.
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