ArticleThe Journal of biological chemistry2011
Biochemical characterization of AtHMA6/PAA1, a chloroplast envelope Cu(I)-ATPase.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Genome-wide identification and expression analysis of HMAs gene family in tobacco under abiotic stress.Scientific reports · 2026Article
- Genome-wide survey of the HMA gene family in wheat (Triticum aestivum L.) and its potential role in cadmium stress.BMC genomics · 2025Article
- Underlying mechanism of Dendrobium huoshanense resistance to lead stress using the quantitative proteomics method.BMC plant biology · 2024Article
- Molecular Mechanisms of Plant Responses to Copper: From Deficiency to Excess.International journal of molecular sciences · 2024Review
- Genome-wide analysis of heavy metal ATPases (HMAs) in Poaceae species and their potential role against copper stress in Triticum aestivum.Scientific reports · 2023Article
- Ectopic Expression ofInternational journal of molecular sciences · 2023Article
- Physiological and Molecular Mechanisms of Plant Responses to Copper Stress.International journal of molecular sciences · 2022Review
- Review
- Article
- Identification of Two Conserved Residues Involved in Copper Release from Chloroplast PIB-1-ATPases.The Journal of biological chemistry · 2016Article
- Regulating Subcellular Metal Homeostasis: The Key to Crop Improvement.Frontiers in plant science · 2016Review
- Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics.Frontiers in plant science · 2016Review
- Structural Insights into the Nucleotide-Binding Domains of the P1B-type ATPases HMA6 and HMA8 from Arabidopsis thaliana.PloS one · 2016Article
- Functional and Biochemical Characterization of Cucumber Genes Encoding Two Copper ATPases CsHMA5.1 and CsHMA5.2.The Journal of biological chemistry · 2015Article
- A sulfur-based transport pathway in Cu+-ATPases.EMBO reports · 2015Article
- HMA6 and HMA8 are two chloroplast Cu+-ATPases with different enzymatic properties.Bioscience reports · 2015Article
- Copper Delivery to Chloroplast Proteins and its Regulation.Frontiers in plant science · 2015Review
- Genome-Wide Analysis and Heavy Metal-Induced Expression Profiling of the HMA Gene Family in Populus trichocarpa.Frontiers in plant science · 2015Article
- Investigating the toxicity, uptake, nanoparticle formation and genetic response of plants to gold.PloS one · 2014Article
- Plastocyanin controls the stabilization of the thylakoid Cu-transporting P-type ATPase PAA2/HMA8 in response to low copper in Arabidopsis.The Journal of biological chemistry · 2012Article
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper is an essential plant micronutrient playing key roles in cellular processes, among them photosynthesis. In Arabidopsis thaliana, copper delivery to chloroplasts, mainly studied by genetic approaches, is thought to involve two P(IB)-type ATPases: AtHMA1 and AtHMA6/PAA1. The lack of biochemical characterization of AtHMA1 and PAA1, and more generally of plant P(IB)-type ATPases, is due to the difficulty of getting high amounts of these membrane proteins in an active form, either from their native environment or after expression in heterologous systems. In this study, we report the first biochemical characterization of PAA1, a plant copper-transporting ATPase. PAA1 produced in Lactococcus lactis is active, forming an aspartyl phosphate intermediate in the presence of ATP and the adequate metal ion. PAA1 can also be phosphorylated using inorganic phosphate in the absence of transition metal. Both phosphorylation types allowed us to demonstrate that PAA1 is activated by monovalent copper ions (and to a lower extent by silver ions) with an apparent affinity in the micromolar range. In agreement with these biochemical data, we also demonstrate that when expressed in yeast, PAA1 induces increased sensitivities to copper and silver. These data provide the first enzymatic characterization of a P(IB-1)-type plant ATPase and clearly identify PAA1 as a high affinity Cu(I) transporter of the chloroplast envelope.
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