ArticleBMC research notes2011
Simultaneous DNA and RNA isolation from brain punches for epigenetics.
Article in BMC research notes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Simultaneous isolation and parallel analysis of genomic DNA and total RNA: A comprehensive approach.Molecular therapy. Methods & clinical development · 2025Article
- Medial preoptic area FoxO1 controls metabolic adaptation in a sexually dimorphic manner.bioRxiv : the preprint server for biology · 2025Article
- Intergenerational accumulation of impairments in maternal behavior following postnatal social stress.Psychoneuroendocrinology · 2017Article
- Social stress during lactation, depressed maternal care, and neuropeptidergic gene expression.Behavioural pharmacology · 2015Article
- Early life social stress induced changes in depression and anxiety associated neural pathways which are correlated with impaired maternal care.Neuropeptides · 2015Article
- Bidirectional rescue of extreme genetic predispositions to anxiety: impact of CRH receptor 1 as epigenetic plasticity gene in the amygdala.Translational psychiatry · 2014Article
- Polycomb binding precedes early-life stress responsive DNA methylation at the Avp enhancer.PloS one · 2014Article
- A-to-I RNA editing does not change with age in the healthy male rat brain.Biogerontology · 2013Article
- Optimized analysis of DNA methylation and gene expression from small, anatomically-defined areas of the brain.Journal of visualized experiments : JoVE · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEpigenetic modifications such as DNA methylation play an important role for gene expression and are regulated by developmental and environmental signals. DNA methylation typically occurs in a highly tissue- and cell-specific manner. This raises a severe challenge when studying discrete, small regions of the brain where cellular heterogeneity is high and tissue quantity limited. Because gene expression and methylation are often tightly linked it appears of interest to compare both parameters in the same sample.
findingsWe present a refined method for the simultaneous extraction of DNA for bisulfite sequencing and RNA for expression analysis from small mouse brain tissue punches. This method can also be easily adapted for other small tissues or cell populations.
conclusionsThe method described herein results in DNA and RNA of a quantity and quality permitting highly reliable bisulfite analysis and quantitative RT-PCR measurements, respectively.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.