ArticlePloS one2011
Phosphorylated CpxR restricts production of the RovA global regulator in Yersinia pseudotuberculosis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Regulation of the Yersinia pseudotuberculosis Type III Secretion System by the CpxAR Two-Component System.Molecular microbiology · 2026Article
- Anti-infective compounds forSustainable microbiology · 2026Review
- The two-component system CpxAR controls biofilm formation by directly regulating the T3SS needle tip protein EseB inApplied and environmental microbiology · 2025Article
- A phosphorylation signal activates genome-wide transcriptional control by BfmR, the global regulator of Acinetobacter resistance and virulence.Nucleic acids research · 2025Article
- Article
- Regulatory role of the Cpx ESR in bacterial behaviours.Virulence · 2024Review
- The CpxAR signaling system confers a fitness advantage for flea gut colonization by the plague bacillus.Journal of bacteriology · 2024Article
- Cpx-signalling innpj antimicrobials and resistance · 2024Article
- The Two-Component System CpxRA Represses Salmonella Pathogenicity Island 2 by Directly Acting on theMicrobiology spectrum · 2022Article
- Cpx-signalling facilitates Hms-dependent biofilm formation by Yersinia pseudotuberculosis.NPJ biofilms and microbiomes · 2022Article
- DissectingJournal of bacteriology · 2021Article
- The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.Virulence · 2019Article
- Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2019Review
- Environmental Regulation of Yersinia Pathophysiology.Frontiers in cellular and infection microbiology · 2016Review
- The Two-Component System CpxRA Negatively Regulates the Locus of Enterocyte Effacement of Enterohemorrhagic Escherichia coli Involving σ(32) and Lon protease.Frontiers in cellular and infection microbiology · 2016Article
- Development and validation of a high-throughput cell-based screen to identify activators of a bacterial two-component signal transduction system.Antimicrobial agents and chemotherapy · 2015Article
- Glycolysis for Microbiome Generation.Microbiology spectrum · 2015Article
- Dynamic interaction between the CpxA sensor kinase and the periplasmic accessory protein CpxP mediates signal recognition in E. coli.PloS one · 2014Article
- Demarcating SurA activities required for outer membrane targeting of Yersinia pseudotuberculosis adhesins.Infection and immunity · 2013Article
- The Cpx stress response system potentiates the fitness and virulence of uropathogenic Escherichia coli.Infection and immunity · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRovA is a global transcriptional regulator of gene expression in pathogenic Yersinia. RovA levels are kept in check by a sophisticated layering of distinct transcriptional and post-transcriptional regulatory mechanisms. In the enteropathogen Y. pseudotuberculosis, we have previously reported that the extracytoplasmic stress sensing CpxA-CpxR two-component regulatory system modulates rovA expression. METHODOLOGY/PRINCIPAL
findingsIn this study, we characterized CpxR phosphorylation (CpxR∼P) in vitro, and determined that phosphorylation was necessary for CpxR to efficiently bind to the PCR-amplified upstream regulatory region of rovA. The precise CpxR∼P binding site was mapped by a nuclease protection assay and directed mutagenesis confirmed that in vivo binding to the rovA promoter inhibits transcription. Reduced RovA production was most pronounced following CpxR∼P accumulation in the Yersinia cytoplasm during chronic Cpx pathway activation and by the indiscriminate phosphodonor action of acetyl phosphate. CONCLUSIONS/SIGNIFICANCE: Cpx pathway activation restricts levels of the RovA global regulator. The regulatory influence of CpxR∼P must therefore extend well beyond periplasmic quality control in the Yersinia envelope, to include genes involved in environmental survival and pathogenicity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.