ArticleJournal of visualized experiments : JoVE2011
Cell-based calcium assay for medium to high throughput screening of TRP channel functions using FlexStation 3.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- TRPA1-dependent and -independent activation by commonly used preservatives.Frontiers in pharmacology · 2023Article
- A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods.Foods (Basel, Switzerland) · 2021Article
- Biological and Molecular Effects ofFrontiers in cellular and infection microbiology · 2021Article
- Establishment of a NanoBiT-Based Cytosolic CaMolecules and cells · 2020Article
- Structure-Dependent Effects of Cinnamaldehyde Derivatives on TRPA1-Induced Serotonin Release in Human Intestinal Cell Models.Journal of agricultural and food chemistry · 2020Article
- Computational design of G Protein-Coupled Receptor allosteric signal transductions.Nature chemical biology · 2020Article
- Computational design of orthogonal membrane receptor-effector switches for rewiring signaling pathways.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Real-Time Signaling Assays Demonstrate Somatostatin Agonist Bias for Ion Channel Regulation in Somatotroph Tumor Cells.Journal of the Endocrine Society · 2018Article
- Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro.Scientific reports · 2018Article
- Zinc Inhibits TRPV1 to Alleviate Chemotherapy-Induced Neuropathic Pain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Computational design of ligand-binding membrane receptors with high selectivity.Nature chemical biology · 2017Article
- Function and regulation of TRPM7, as well as intracellular magnesium content, are altered in cells expressing ΔF508-CFTR and G551D-CFTR.Cellular and molecular life sciences : CMLS · 2016Article
- High throughput screening technologies for ion channels.Acta pharmacologica Sinica · 2016Review
- Retinoids activate the irritant receptor TRPV1 and produce sensory hypersensitivity.The Journal of clinical investigation · 2013Article
- Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2C receptors.BMC neuroscience · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. It is ideal for medium- to high-throughput screens in academic settings. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time to monitor fluorescence changes of a variety of fluorescent reagents. For example, FlexStation 3 has been used to study the function of Ca(2+)-permeable ion channels and G-protein coupled receptors by measuring the changes of intracellular free Ca(2+) levels. Transient receptor potential (TRP) channels are a large family of nonselective cation channels that play important roles in many physiological and pathophysiological functions. Most of the TRP channels are calcium permeable and induce calcium influx upon activation. In this video, we demonstrate the application of FlexStation 3 to study the pharmacological profile of the TRPA1 channel, a molecular sensor for numerous noxious stimuli. HEK293 cells transiently or stably expressing human TRPA1 channels, grown in 96-well plates, are loaded with a Ca(2+)-sensitive fluorescent dye, Fluo-4, and real-time fluorescence changes in these cells are measured before and during the application of a TRPA1 agonist using the FLEX mode of the FlexStation 3. The effect of a putative TRPA1 antagonist was also examined. Data are transferred from the SoftMax Pro software to construct concentration-response relationships of TRPA1 activators and inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.