ArticleJournal of molecular biology2011
Light-induced conformational changes in full-length Arabidopsis thaliana cryptochrome.
Article in Journal of molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 74 citations in OpenAlex.
- Decoding darkness by seeking photoreceptor functions with and without light.Nature plants · 2026Review
- The Quantum Environment in Cryptochrome Enhances Light Absorption of FAD.bioRxiv : the preprint server for biology · 2026Article
- Disordered but rhythmic-the role of intrinsic protein disorder in eukaryotic circadian timing.FEBS letters · 2026Review
- Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025Review
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- 'Seeing' the electromagnetic spectrum: spotlight on the cryptochrome photocycle.Frontiers in plant science · 2024Review
- Dimerization of European Robin Cryptochrome 4a.The journal of physical chemistry. B · 2023Article
- Molecular Cloning and Expression Analysis of the Cryptochrome GenePlants (Basel, Switzerland) · 2022Article
- Cryptochrome expression in avian UV cones: revisiting the role of CRY1 as magnetoreceptor.Scientific reports · 2021Article
- Activation mechanism ofScience advances · 2021Article
- Structural insights into photoactivation of plant Cryptochrome-2.Communications biology · 2021Article
- Plant Cryptochromes Illuminated: A Spectroscopic Perspective on the Mechanism.Frontiers in chemistry · 2021Review
- Article
- Blue-Light Receptors for Optogenetics.Chemical reviews · 2018Review
- Blue-light induced biosynthesis of ROS contributes to the signaling mechanism of Arabidopsis cryptochrome.Scientific reports · 2017Article
- Structural disorder in plant proteins: where plasticity meets sessility.Cellular and molecular life sciences : CMLS · 2017Review
- Hyperactivity of theThe Journal of biological chemistry · 2017Article
- Cryptochromes Orchestrate Transcription Regulation of Diverse Blue Light Responses in Plants.Photochemistry and photobiology · 2017Review
- Inhomogeneous ensembles of radical pairs in chemical compasses.Scientific reports · 2016Article
- Essential Role of an Unusually Long-lived Tyrosyl Radical in the Response to Red Light of the Animal-like Cryptochrome aCRY.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Cryptochromes (CRYs) are widespread flavoproteins with homology to photolyases (PHRs), a class of blue-light-activated DNA repair enzymes. Unlike PHRs, both plant and animal CRYs have a C-terminal domain. This cryptochrome C-terminal (CCT) domain mediates interactions with other proteins, while the PHR-like domain converts light energy into a signal via reduction and radical formation of the flavin adenine dinucleotide cofactor. However, the mechanism by which the PHR-like domain regulates the CCT domain is not known. Here, we applied the pulsed-laser-induced transient grating method to detect conformational changes induced by blue-light excitation of full-length Arabidopsis thaliana cryptochrome 1 (AtCRY1). A significant reduction in the diffusion coefficient of AtCRY1 was observed upon photoexcitation, indicating that a large conformational change occurs in this monomeric protein. AtCRY1 containing a single mutation (W324F) that abolishes an intra-protein electron transfer cascade did not exhibit this conformational change. Moreover, the conformational change was much reduced in protein lacking the CCT domain. Thus, we conclude that the observed large conformational changes triggered by light excitation of the PHR-like domain result from C-terminal domain rearrangement. This inter-domain modulation would be critical for CRYs' ability to transduce a blue-light signal into altered protein-protein interactions for biological activity. Lastly, we demonstrate that the transient grating technique provides a powerful method for the direct observation and understanding of photoreceptor dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.