ArticleThe EMBO journal2011
RUNX1 regulates the CD34 gene in haematopoietic stem cells by mediating interactions with a distal regulatory element.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Optimizing in vitro T cell differentiation by using induced pluripotent stem cells with GFP-RUNX1 and mCherry-TCF7 labelling.Cell proliferation · 2024Article
- Spatiotemporal Epigenetic Control of the Histone Gene Chromatin Landscape during the Cell Cycle.Critical reviews in eukaryotic gene expression · 2023Article
- Epigenetic-Mediated Regulation of Gene Expression for Biological Control and Cancer: Cell and Tissue Structure, Function, and Phenotype.Results and problems in cell differentiation · 2022Review
- Myeloid lncRNA LOUP mediates opposing regulatory effects of RUNX1 and RUNX1-ETO in t(8;21) AML.Blood · 2021Article
- Runx Transcription Factors in T Cells-What Is Beyond Thymic Development?Frontiers in immunology · 2021Review
- The epigenetic regulator SIRT6 protects the liver from alcohol-induced tissue injury by reducing oxidative stress in mice.Journal of hepatology · 2019Article
- Higher order genomic organization and epigenetic control maintain cellular identity and prevent breast cancer.Genes, chromosomes & cancer · 2019Review
- Transcriptional regulation of Hepatic Stellate Cell activation in NASH.Scientific reports · 2019Article
- Article
- The Co-operation of RUNX1 with LDB1, CDK9 and BRD4 Drives Transcription Factor Complex Relocation During Haematopoietic Specification.Scientific reports · 2018Article
- RUNX1 contributes to higher-order chromatin organization and gene regulation in breast cancer cells.Biochimica et biophysica acta · 2016Article
- CD34 expression modulates tube-forming capacity and barrier properties of peripheral blood-derived endothelial colony-forming cells (ECFCs).Angiogenesis · 2016Article
- RUNX1B Expression Is Highly Heterogeneous and Distinguishes Megakaryocytic and Erythroid Lineage Fate in Adult Mouse Hematopoiesis.PLoS genetics · 2016Article
- Chromosomes at Work: Organization of Chromosome Territories in the Interphase Nucleus.Journal of cellular biochemistry · 2016Article
- Interplay between Transcription Factors and the Epigenome: Insight from the Role of RUNX1 in Leukemia.Frontiers in immunology · 2015Review
- Article
- Shared transcription factors contribute to distinct cell fates.Transcription · 2014Article
- Sustained PU.1 levels balance cell-cycle regulators to prevent exhaustion of adult hematopoietic stem cells.Molecular cell · 2013Article
- MYCN transgenic zebrafish model with the characterization of acute myeloid leukemia and altered hematopoiesis.PloS one · 2013Article
- RUNX1 reshapes the epigenetic landscape at the onset of haematopoiesis.The EMBO journal · 2012Article
Corrections and comments
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Authors and funding
19 authors at 11 institutions in 6 countries.
Funding
Abstract
The transcription factor RUNX1 is essential to establish the haematopoietic gene expression programme; however, the mechanism of how it activates transcription of haematopoietic stem cell (HSC) genes is still elusive. Here, we obtained novel insights into RUNX1 function by studying regulation of the human CD34 gene, which is expressed in HSCs. Using transgenic mice carrying human CD34 PAC constructs, we identified a novel downstream regulatory element (DRE), which is bound by RUNX1 and is necessary for human CD34 expression in long-term (LT)-HSCs. Conditional deletion of Runx1 in mice harbouring human CD34 promoter-DRE constructs abrogates human CD34 expression. We demonstrate by chromosome conformation capture assays in LT-HSCs that the DRE physically interacts with the human CD34 promoter. Targeted mutagenesis of RUNX binding sites leads to perturbation of this interaction and decreased human CD34 expression in LT-HSCs. Overall, our in vivo data provide novel evidence about the role of RUNX1 in mediating interactions between distal and proximal elements of the HSC gene CD34.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.