ArticleInvestigative ophthalmology & visual science2011
Identification of the muscarinic acetylcholine receptor subtype mediating cholinergic vasodilation in murine retinal arterioles.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
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- Serum cholinesterase is associated with incident diabetic retinopathy: the Shanghai Nicheng cohort study.Nutrition & metabolism · 2023Article
- Effects of Resveratrol on Vascular Function in Retinal Ischemia-Reperfusion Injury.Antioxidants (Basel, Switzerland) · 2023Article
- Intraocular Pressure-Induced Endothelial Dysfunction of Retinal Blood Vessels Is Persistent, but Does Not Trigger Retinal Ganglion Cell Loss.Antioxidants (Basel, Switzerland) · 2022Article
- Effect of Intracameral Carbachol and Epinephrine Use on Choroidal Thickness After Uncomplicated Phacoemulsification.Medical science monitor : international medical journal of experimental and clinical research · 2022Article
- Methods to measure blood flow and vascular reactivity in the retina.Frontiers in medicine · 2022Review
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- Mechanical strength and hydrostatic testing of VIVO adhesive in sutureless microsurgical anastomoses: an ex vivo study.Scientific reports · 2021Article
- Muscarinic Acetylcholine Receptors in the Retina-Therapeutic Implications.International journal of molecular sciences · 2021Review
- Ablation of lysozyme M-positive cells prevents aircraft noise-induced vascular damage without improving cerebral side effects.Basic research in cardiology · 2021Article
- Role of Choline in Ocular Diseases.International journal of molecular sciences · 2021Review
- Optogenetic Stimulation of Cholinergic Amacrine Cells Improves Capillary Blood Flow in Diabetic Retinopathy.Investigative ophthalmology & visual science · 2020Article
- Roles of vacuolar HInternational journal of clinical and experimental pathology · 2020Article
- The MScientific reports · 2019Article
- Apolipoprotein E Deficiency Causes Endothelial Dysfunction in the Mouse Retina.Oxidative medicine and cellular longevity · 2019Article
- Elevated Intraocular Pressure Causes Abnormal Reactivity of Mouse Retinal Arterioles.Oxidative medicine and cellular longevity · 2019Article
- Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo.Journal of visualized experiments : JoVE · 2018Article
- All muscarinic acetylcholine receptors (MScientific reports · 2017Article
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
Abstract
purposeTo identify the muscarinic acetylcholine receptor subtype that mediates cholinergic vasodilation in murine retinal arterioles.
methodsMuscarinic receptor gene expression was determined in murine retinal arterioles using real-time PCR. To assess the functional relevance of muscarinic receptors for mediating vascular responses, retinal vascular preparations from muscarinic receptor-deficient mice were studied in vitro. Changes in luminal arteriole diameter in response to muscarinic and nonmuscarinic vasoactive substances were measured by video microscopy.
resultsOnly mRNA for the M(3) receptor was detected in retinal arterioles. Thus, M(3) receptor-deficient mice (M3R(-/-)) and respective wild-type controls were used for functional studies. Acetylcholine concentration-dependently dilated retinal arterioles from wild-type mice. In contrast, vasodilation to acetylcholine was almost completely abolished in retinal arterioles from M3R(-/-) mice, whereas responses to the nitric oxide (NO) donor nitroprusside were retained. Carbachol, an acetylcholinesterase-resistant analog of acetylcholine, also evoked dilation in retinal arterioles from wild-type, but not from M3R(-/-), mice. Vasodilation responses from wild-type mice to acetylcholine were negligible after incubation with the non-subtype-selective muscarinic receptor blocker atropine or the NO synthase inhibitor N(ω)-nitro-L-arginine methyl ester, and were even reversed to contraction after endothelial damage with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate.
conclusionsThese findings provide evidence that endothelial M(3) receptors mediate cholinergic vasodilation in murine retinal arterioles via activation of NO synthase.
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