ArticleApplied and environmental microbiology2011
Improvement in the thermostability of D-psicose 3-epimerase from Agrobacterium tumefaciens by random and site-directed mutagenesis.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 70 citations in OpenAlex.
- Production, purification, characterization, and safety evaluation of constructed recombinant D-psicose 3-epimerase.Microbial cell factories · 2024Article
- Growth-Coupled Evolutionary Pressure Improving Epimerases for D-Allulose Biosynthesis Using a Biosensor-Assisted In Vivo Selection Platform.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Optimization of Ultrahigh-Throughput Screening Assay for Protein Engineering of d-Allulose 3-Epimerase.Biomolecules · 2022Article
- The Characterization of a Novel D-allulose 3-Epimerase fromFoods (Basel, Switzerland) · 2022Article
- Structural Analysis and Construction of a Thermostable Antifungal Chitinase.Applied and environmental microbiology · 2022Article
- Characterization of a Recombinant D-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in D-Allulose Production.Molecular biotechnology · 2021Article
- An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes.World journal of microbiology & biotechnology · 2021Review
- D-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat-stability and noteworthy potential of D-fructose epimerization.Microbial cell factories · 2021Article
- A Novel d-Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d-Allulose Production by Bacillus subtilis Whole-Cell Catalysis.Applied and environmental microbiology · 2020Article
- Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases.Biotechnology for biofuels · 2020Article
- High Conversion of D-Fructose into D-Allulose by Enzymes Coupling with an ATP Regeneration System.Molecular biotechnology · 2019Article
- Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose.Microbial cell factories · 2019Article
- Biosensor-based enzyme engineering approach applied to psicose biosynthesis.Synthetic biology (Oxford, England) · 2019Article
- Bioconversion of D-glucose to D-psicose with immobilized D-xylose isomerase and D-psicose 3-epimerase on Saccharomyces cerevisiae spores.Journal of industrial microbiology & biotechnology · 2015Article
- From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.International journal of molecular sciences · 2013Review
- Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704.PloS one · 2013Article
- Enzymes for the biocatalytic production of rare sugars.Journal of industrial microbiology & biotechnology · 2012Review
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The S213C, I33L, and I33L S213C variants of D-psicose 3-epimerase from Agrobacterium tumefaciens, which were obtained by random and site-directed mutagenesis, displayed increases of 2.5, 5, and 7.5°C in the temperature for maximal enzyme activity, increases of 3.3-, 7.2-, and 29.9-fold in the half-life at 50°C, and increases of 3.1, 4.3, and 7.6°C in apparent melting temperature, respectively, compared with the wild-type enzyme. Molecular modeling suggests that the improvement in thermostability in these variants may have resulted from increased putative hydrogen bonds and formation of new aromatic stacking interactions. The immobilized wild-type enzyme with and without borate maintained activity for 8 days at a conversion yield of 70% (350 g/liter psicose) and for 16 days at a conversion yield of 30% (150 g/liter psicose), respectively. After 8 or 16 days, the enzyme activity gradually decreased, and the conversion yields with and without borate were reduced to 22 and 9.6%, respectively, at 30 days. In contrast, the activities of the immobilized I33L S213C variant with and without borate did not decrease during the operation time of 30 days. These results suggest that the I33L S213C variant may be useful as an industrial producer of D-psicose.
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