ArticleMicrobiology (Reading, England)2011
Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa.
Article in Microbiology (Reading, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 56 citations in OpenAlex.
- The type VI secretion system and associated effector proteins.Nature reviews. Microbiology · 2026Review
- The Anti-Listeria Activity ofPathogens (Basel, Switzerland) · 2023Article
- Type VI Secretion Systems: Environmental and Intra-host Competition of Vibrio cholerae.Advances in experimental medicine and biology · 2023Article
- Combining bacteriophage and vancomycin is efficacious against MRSA biofilm-like aggregates formed in synovial fluid.Frontiers in medicine · 2023Article
- Distribution of genes related to Type 6 secretion system and lipooligosaccharide that induced ganglioside mimicry amongGut pathogens · 2020Article
- The Pseudomonas aeruginosa T6SS Delivers a Periplasmic Toxin that Disrupts Bacterial Cell Morphology.Cell reports · 2019Article
- Structure and Activity of the Type VI Secretion System.Microbiology spectrum · 2019Review
- Abundance of bacterial Type VI secretion system components measured by targeted proteomics.Nature communications · 2019Article
- Structural basis for inhibition of a response regulator of σGenes & development · 2019Article
- Crosstalk Between Type VI Secretion System and Mobile Genetic Elements.Frontiers in molecular biosciences · 2019Review
- Baseplate Component TssK and Spatio-Temporal Assembly of T6SS inFrontiers in microbiology · 2019Article
- Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end.The EMBO journal · 2018Article
- Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex.Nature microbiology · 2017Article
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- TssA forms a gp6-like ring attached to the type VI secretion sheath.The EMBO journal · 2016Article
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- The Type VI Secretion System in Escherichia coli and Related Species.EcoSal Plus · 2016Review
- Type VI secretion system: secretion by a contractile nanomachine.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Bacterial pathogens use a range of protein secretion systems to colonize their host. One recent addition to this arsenal is the type VI secretion system (T6SS), which is found in many Gram-negative bacteria. The T6SS involves 12-15 components, including a ClpV-like AAA(+) ATPase. Moreover, the VgrG and Hcp components have been proposed to form a puncturing device, based on structural similarity to the tail spike components gp5/gp27 and the tail tube component gp19 of the T4 bacteriophage, respectively. Another T6SS component shows similarity to a T4 phage protein, namely gp25. The gp25 protein has been proposed to have lysozyme activity. Other T6SS components do not exhibit obvious similarity to characterized T4 phage components. The genome of Pseudomonas aeruginosa contains three T6SS gene clusters. In each cluster a gene encoding a putative member of the gp25-like protein family was identified, which we called HsiF. We confirmed this similarity by analysing the structure of the P. aeruginosa HsiF proteins using secondary and tertiary structure prediction tools. We demonstrated that HsiF1 is crucial for the T6SS-dependent secretion of Hcp and VgrG. Importantly, lysozyme activity of HsiF proteins was not detectable, and we related this observation to the demonstration that HsiF1 localizes to the cytoplasm of P. aeruginosa. Finally, our data showed that a conserved glutamate, predicted to be required for proper HsiF folding, is essential for its function. In conclusion, our data confirm the central role of HsiF in the T6SS mechanism, provide information on the predicted HsiF structure, and call for reconsideration of the function of gp25-like proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.