ArticleProceedings of the National Academy of Sciences of the United States of America2011
Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host range expansion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 126 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
126 citing papers in PubMed.
- A Nutrient-Responsive LuxR Regulator Orchestrates Effector Gene Expression Across the Legionella Genus.Molecular microbiology · 2026Article
- Systematic discovery of novel phosphoinositide-binding effectors inMicrobiology spectrum · 2026Article
- Major type IV pilin ofmBio · 2026Article
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Systematic Discovery of Novel Phosphoinositide-Binding Effectors inbioRxiv : the preprint server for biology · 2025Article
- The Legionella pneumophila Dot/Icm Type IV Secretion System is Structurally and Functionally Resilient in Absence of Species-specific Proteins Dis2 and Dis3.Journal of molecular biology · 2025Article
- Single-cell analysis of genetically minimized Salmonella reveals effector gene cooperation in vivo.Nature microbiology · 2025Article
- The discovery of aScience advances · 2025Article
- Phosphatidic acid production on the vacuole harboringProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Host-Specific Adaptation of Legionella pneumophila to Single and Multiple Hosts.Molecular biology and evolution · 2025Article
- Modulation of host ATP levels by secreted bacterial effectors.Nature communications · 2025Article
- Article
- Subversion of the host endocytic pathway by Legionella pneumophila-mediated ubiquitination of Rab5.The Journal of cell biology · 2025Article
- ThemSphere · 2025Article
- Legionella uses host Rab GTPases and BAP31 to create a unique ER niche.Cell reports · 2024Article
- Review
- InSeq analysis of definedmBio · 2024Article
- Phosphorylation of caspases by a bacterial kinase inhibits host programmed cell death.Nature communications · 2024Article
- A random mutagenesis screen enriched for missense mutations in bacterial effector proteins.G3 (Bethesda, Md.) · 2024Article
- Review
66 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Legionella pneumophila is a bacterial pathogen of amoebae and humans. Intracellular growth requires a type IVB secretion system that translocates at least 200 different proteins into host cells. To distinguish between proteins necessary for growth in culture and those specifically required for intracellular replication, a screen was performed to identify genes necessary for optimal growth in nutrient-rich medium. Mapping of these genes revealed that the L. pneumophila chromosome has a modular architecture consisting of several large genomic islands that are dispensable for growth in bacteriological culture. Strains lacking six of these regions, and thus 18.5% of the genome, were viable but required secondary point mutations for optimal growth. The simultaneous deletion of five of these genomic loci had no adverse effect on growth of the bacterium in nutrient-rich media. Remarkably, this minimal genome strain, which lacked 31% of the known substrates of the type IVB system, caused only marginal defects in intracellular growth within mouse macrophages. In contrast, deletion of single regions reduced growth within amoebae. The importance of individual islands, however, differed among amoebal species. The host-specific requirements of these genomic islands support a model in which the acquisition of foreign DNA has broadened the L. pneumophila host range.
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