ArticleAtherosclerosis2011
Molecular imaging with optical coherence tomography using ligand-conjugated microparticles that detect activated endothelial cells: rational design through target quantification.
Article in Atherosclerosis, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- In Vivo PET Detection of Lung Micrometastasis in Mice by Targeting Endothelial VCAM-1 Using a Dual-Contrast PET/MRI Probe.International journal of molecular sciences · 2024Article
- Epigenetic basis of diabetic vasculopathy.Frontiers in endocrinology · 2022Review
- A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels.Scientific reports · 2019Article
- Molecular Imaging of the Heart.Comprehensive Physiology · 2019Review
- Monocyte and Macrophage Dynamics in the Cardiovascular System: JACC Macrophage in CVD Series (Part 3).Journal of the American College of Cardiology · 2018Review
- Optimization of molecularly targeted MRI in the brain: empirical comparison of sequences and particles.International journal of nanomedicine · 2018Article
- Diagnostic and prognostic utility of non-invasive imaging in diabetes management.World journal of diabetes · 2015Review
- Molecular magnetic resonance imaging of angiogenesis in vivo using polyvalent cyclic RGD-iron oxide microparticle conjugates.Theranostics · 2015Article
- Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice.BioMed research international · 2015Article
- Unmasking Silent Endothelial Activation in the Cardiovascular System Using Molecular Magnetic Resonance Imaging.Theranostics · 2015Article
- Magnetomotive optical coherence tomography for the assessment of atherosclerotic lesions using αvβ3 integrin-targeted microspheres.Molecular imaging and biology · 2014Article
- Molecular magnetic resonance imaging of brain-immune interactions.Frontiers in cellular neuroscience · 2014Review
- Molecular imaging of inflammation in atherosclerosis.Theranostics · 2013Review
- Detection and minimally invasive treatment of early squamous lung cancer.Therapeutic advances in medical oncology · 2013Article
- Exogenous microparticles of iron oxide bind to activated endothelial cells but, unlike monocytes, do not trigger an endothelial response.Theranostics · 2013Article
- Label-Free, Longitudinal Visualization of PDT Response In Vitro with Optical Coherence Tomography.Israel journal of chemistry · 2012Article
- Metal imaging in neurodegenerative diseases.Metallomics : integrated biometal science · 2012Review
- Molecular imaging of atherosclerosis for improving diagnostic and therapeutic development.Circulation research · 2012Review
- A leukocyte-mimetic magnetic resonance imaging contrast agent homes rapidly to activated endothelium and tracks with atherosclerotic lesion macrophage content.Arteriosclerosis, thrombosis, and vascular biology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesOptical coherence tomography (OCT) is a high resolution imaging technique used to assess superficial atherosclerotic plaque morphology. Utility of OCT may be enhanced by contrast agents targeting molecular mediators of inflammation. METHODS AND
resultsMicroparticles of iron oxide (MPIO; 1 and 4.5 μm diameter) in suspension were visualized and accurately quantified using a clinical optical coherence tomography system. Bound to PECAM-1 on a plane of cultured endothelial cells under static conditions, 1 μm MPIO were also readily detected by OCT. To design a molecular contrast probe that would bind activated endothelium under conditions of shear stress, we quantified the expression (basal vs. TNF-activated; molecules μm(-2)) of VCAM-1 (not detected vs. 16 ± 1); PECAM-1 (132 ± 6 vs. 198 ± 10) and E-selectin (not detected vs. 46 ± 0.6) using quantitative flow cytometry. We then compared the retention of antibody-conjugated MPIO targeting each of these molecules plus a combined VCAM-1 and E-selectin (E+V) probe across a range of physiologically relevant shear stresses. E+V MPIO were consistently retained with highest efficiency (P < 0.001) and at a density that provided conspicuous contrast effects on OCT pullback.
conclusionMicroparticles of iron oxide were detectable using a clinical OCT system. Assessment of binding under flow conditions recommended an approach that targeted both E-selectin and VCAM-1. Bound to HUVEC under conditions of flow, targeted 1 μm E+V MPIO were readily identified on OCT pullback. Molecular imaging with OCT may be feasible in vivo using antibody targeted MPIO.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.