ArticleNucleic acids research1990
Characterisation of the DNA binding domain of the yeast RAP1 protein.
Article in Nucleic acids research, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 70 citations in OpenAlex.
- Telomere length regulation by Rif1 protein fromeLife · 2022Article
- Functional duplication of Rap1 in methylotrophic yeasts.Scientific reports · 2019Article
- Identification of a transcriptional activation domain in yeast repressor activator protein 1 (Rap1) using an altered DNA-binding specificity variant.The Journal of biological chemistry · 2017Article
- Molecular dissection of the genetic mechanisms that underlie expression conservation in orthologous yeast ribosomal promoters.Genome research · 2014Article
- The orientation of the C-terminal domain of the Saccharomyces cerevisiae Rap1 protein is determined by its binding to DNA.Nucleic acids research · 2012Article
- Molecular Dynamics Simulation of Rap1 Myb-type domain in Saccharomyces cerevisiae.Bioinformation · 2012Article
- In vivo analysis of functional regions within yeast Rap1p.Molecular and cellular biology · 1999Article
- Synergistic operation of the CAR2 (Ornithine transaminase) promoter elements in Saccharomyces cerevisiae.Journal of bacteriology · 1999Article
- Article
- The yeast telomere length counting machinery is sensitive to sequences at the telomere-nontelomere junction.Molecular and cellular biology · 1999Article
- Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The C terminus of the major yeast telomere binding protein Rap1p enhances telomere formation.Molecular and cellular biology · 1998Article
- The C-terminal silencing domain of Rap1p is essential for the repression of ribosomal protein genes in response to a defect in the secretory pathway.Nucleic acids research · 1998Article
- Genetic analysis of Rap1p/Sir3p interactions in telomeric and HML silencing in Saccharomyces cerevisiae.Genetics · 1996Article
- Article
- Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae).Current genetics · 1995Review
- The carboxy termini of Sir4 and Rap1 affect Sir3 localization: evidence for a multicomponent complex required for yeast telomeric silencing.The Journal of cell biology · 1995Article
- Extra telomeres, but not internal tracts of telomeric DNA, reduce transcriptional repression at Saccharomyces telomeres.Genetics · 1995Article
- Mutational analysis defines a C-terminal tail domain of RAP1 essential for Telomeric silencing in Saccharomyces cerevisiae.Genetics · 1994Article
- Promotion of parallel DNA quadruplexes by a yeast telomere binding protein: a circular dichroism study.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 827 amino acid yeast RAP1 protein interacts with DNA to regulate gene expression at numerous unrelated loci in the yeast genome. By a combination of amino, carboxy and internal deletions, we have defined an internal 235 amino acid fragment of the yeast RAP1 protein that can bind efficiently to the RAP1 binding site of the PGK Upstream Activation Sequence (UAS). This domain spans residues 361 to 596 of the full length protein and lacks any homology to the DNA binding 'zinc finger' or 'helix-turn-helix' structural motifs. All the RAP1 binding sites we have tested bind domain 361-596, arguing that RAP1 binds all its chromosomal sites via this domain. The domain could not be further reduced in size suggesting that it represents the minimal functional DNA binding domain. The relevance of potential regions of secondary structure within the minimal binding domain is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.