Evidence map›Paper›PMID 21871505›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2012

EGFR-targeted stearoyl gemcitabine nanoparticles show enhanced anti-tumor activity.

Michael A Sandoval, Brian R Sloat, Dharmika S P Lansakara-P, Amit Kumar, B Leticia Rodriguez, Kaoru Kiguchi, John Digiovanni, Zhengrong Cui

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

  1. Pooled it
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  5. Nanomedicine in cancer therapy.Signal transduction and targeted therapy · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Michael A SandovalPharmaceutics Division, The University of Texas at Austin, College of Pharmacy, Austin, Texas 78712, USA.
Brian R Sloat
Dharmika S P Lansakara-P
Amit Kumar
B Leticia Rodriguez
Kaoru Kiguchi
John Digiovanni
Zhengrong Cui
The University of Texas at Austin · USAustin College · US

Funding

Overcoming pancreatic tumor resistance to gemcitabineR01CA135274 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI CUI, ZHENGRONG · 2008 to 2017
$2.8M
NCI NIH HHS CA135274NCI NIH HHS R01 CA135274
6 · The paper itself

Abstract

Previously, it was shown that a novel 4-(N)-stearoyl gemcitabine nanoparticle formulation was more effective than gemcitabine hydrochloride in controlling the growth of model mouse or human tumors pre-established in mice. In the present study, the feasibility of targeting the stearoyl gemcitabine nanoparticles (GemC18-NPs) into tumor cells that over-express epidermal growth factor receptor (EGFR) to more effectively control tumor growth was evaluated. EGFR is over-expressed in a variety of tumor cells, and EGF is a known natural ligand of EGFR. Recombinant murine EGF was conjugated onto the GemC18-NPs. The ability of the EGF to target the GemC18-NPs to human breast adenocarcinoma cells that expressed different levels of EGFR was evaluated in vitro and in vivo. In culture, the extent to which the EGF-conjugated GemC18-NPs were taken up by tumor cells was correlated to the EGFR density on the tumor cells, whereas the uptake of untargeted GemC18-NPs exhibited no difference among those same cell lines. The relative cytotoxicity of the EGF-conjugated GemC18-NPs to tumor cells in culture was correlated to EGFR expression as well. In vivo, EGFR-over-expressing MDA-MB-468 tumors in mice treated with the EGF-conjugated GemC18-NPs grew significantly slower than in mice treated with untargeted GemC18-NPs, likely due to that the EGF-GemC18-NPs were more anti-proliferative, anti-angiogenic, and pro-apoptotic. Fluorescence intensity data from ex vivo imaging showed that the EGF on the nanoparticles helped increase the accumulation of the GemC18-NPs into MDA-MB-468 tumors pre-established in mice by more than 2-fold as compared to the un-targeted GemC18-NPs. In conclusion, active targeting of the GemC18-NPs into EGFR-over-expressed tumors can further enhance their anti-tumor activity.

Indexed as

AnimalsAntimetabolites, AntineoplasticCell Line, TumorDeoxycytidineEpidermal Growth FactorErbB ReceptorsGemcitabineHumansMiceMice, NudeMolecular Targeted TherapyNanoparticlesNeoplasmsOvalbuminTumor BurdenAntimetabolites, AntineoplasticDeoxycytidineEpidermal Growth FactorErbB ReceptorsGemcitabineOvalbumin

Identifiers

PMID21871505
PMCPMC3253326
OpenAlexW2025869556

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.