ArticleRespiratory research2011
Immunodetection of occult eosinophils in lung tissue biopsies may help predict survival in acute lung injury.
Article in Respiratory research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Trial
- Significance of eosinophil accumulation in the thrombus and decrease in peripheral blood in patients with acute coronary syndrome.Coronary artery disease · 2015Trial
- Review
- Antibiotic use during influenza infection augments lung eosinophils that impair immunity against secondary bacterial pneumonia.The Journal of clinical investigation · 2024Article
- ARDS Clinical Practice Guideline 2021.Journal of intensive care · 2022Article
- Identification of key genes related to immune cells in patients with gram-negative sepsis based on weighted gene co-expression network analysis.Annals of translational medicine · 2022Article
- Preexisting Trichinella spiralis infection attenuates the severity of Pseudomonas aeruginosa-induced pneumonia.PLoS neglected tropical diseases · 2022Article
- Recent advances of eosinophils and its correlated diseases.Frontiers in public health · 2022Review
- Predictive Value of the Baseline and Early Changes in Blood Eosinophils for Short-Term Mortality in Patients with Acute Respiratory Distress Syndrome.Journal of inflammation research · 2022Article
- The role of eosinophils in sepsis and acute respiratory distress syndrome: a scoping review.Canadian journal of anaesthesia = Journal canadien d'anesthesie · 2021Article
- IL-33-mediated Eosinophilia Protects against Acute Lung Injury.American journal of respiratory cell and molecular biology · 2021Article
- Assessment of Lung Eosinophils In Situ Using Immunohistological Staining.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Blood eosinophils and mortality in patients with acute respiratory distress syndrome: A propensity score matching analysis.World journal of emergency medicine · 2021Article
- Primary tumors from mucosal barrier organs drive unique eosinophil infiltration patterns and clinical associations.Oncoimmunology · 2020Article
- A new dawn for eosinophils in the tumour microenvironment.Nature reviews. Cancer · 2020Review
- Assessment of eosinophils in gastrointestinal inflammatory disease of dogs.Journal of veterinary internal medicine · 2018Article
- Revisiting the NIH Taskforce on the Research needs of Eosinophil-Associated Diseases (RE-TREAD).Journal of leukocyte biology · 2018Review
- Predictors of diffuse alveolar damage in patients with acute respiratory distress syndrome: a retrospective analysis of clinical autopsies.Critical care (London, England) · 2017Article
- Lung-resident eosinophils represent a distinct regulatory eosinophil subset.The Journal of clinical investigation · 2016Article
- Tumor eosinophil infiltration and improved survival of colorectal cancer patients: Iowa Women's Health Study.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2016Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAcute lung injury (ALI) is a serious respiratory disorder for which therapy is primarily supportive once infection is excluded. Surgical lung biopsy may rule out other diagnoses, but has not been generally useful for therapy decisions or prognosis in this setting. Importantly, tissue and peripheral blood eosinophilia, the hallmarks of steroid-responsive acute eosinophilic pneumonia, are not commonly linked with ALI. We hypothesized that occult eosinophilic pneumonia may explain better outcomes for some patients with ALI.
methodsImmunohistochemistry using a novel monoclonal antibody recognizing eosinophil peroxidase (EPX-mAb) was used to assess intrapulmonary eosinophil accumulation/degranulation. Lung biopsies from ALI patients (n = 20) were identified following review of a pathology database; 45% of which (i.e., 9/20) displayed classical diffuse alveolar damage (ALI-DAD). Controls were obtained from uninvolved tissue in patients undergoing lobectomy for lung cancer (n = 10). Serial biopsy sections were stained with hematoxylin and eosin (H&E) and subjected to EPX-mAb immunohistochemistry.
resultsEPX-mAb immunohistochemistry provided a >40-fold increased sensitivity to detect eosinophils in the lung relative to H&E stained sections. This increased sensitivity led to the identification of higher numbers of eosinophils in ALI patients compared with controls; differences using H&E staining alone were not significant. Clinical assessments showed that lung infiltrating eosinophil numbers were higher in ALI patients that survived hospitalization compared with non-survivors. A similar conclusion was reached quantifying eosinophil degranulation in each biopsy.
conclusionThe enhanced sensitivity of EPX-mAb immunohistochemistry uniquely identified eosinophil accumulation/degranulation in patients with ALI relative to controls. More importantly, this method was a prognostic indicator of patient survival. These observations suggest that EPX-mAb immunohistochemistry may represent a diagnostic biomarker identifying a subset of ALI patients with improved clinical outcomes.
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