ArticleBioconjugate chemistry2011
Improved templated fluorogenic probes enhance the analysis of closely related pathogenic bacteria by microscopy and flow cytometry.
Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- RNA Probes for Visualization of Sarcin/ricin Loop Depurination without Background Fluorescence.Chemistry, an Asian journal · 2022Article
- Bioorthogonal Reactions of Triarylphosphines and Related Analogues.Chemical reviews · 2021Review
- Development of a fixation-free fluorescenceBiology methods & protocols · 2020Article
- Magnetic Field-Activated Sensing of mRNA in Living Cells.Journal of the American Chemical Society · 2017Article
- Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.Frontiers in microbiology · 2017Review
- Bioorthogonal tetrazine-mediated transfer reactions facilitate reaction turnover in nucleic acid-templated detection of microRNA.Journal of the American Chemical Society · 2014Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Simplified confocal microscope for counting particles at low concentrations.The Review of scientific instruments · 2013Article
- Oligonucleotide-templated reactions for sensing nucleic acids.Molecules (Basel, Switzerland) · 2012Review
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Authors and funding
2 authors.
Funding
Abstract
Templated fluorescence activation has recently emerged as a promising molecular approach to detect and differentiate nucleic acid sequences in vitro and in cells. Here, we describe the application of a reductive quencher release strategy to the taxonomic analysis of Gram-negative bacteria by targeting a single nucleotide difference in their 16S rRNA in a two-color assay. For this purpose, it was necessary to develop a release linker containing a quencher suitable for red and near-infrared fluorophores, and to improve methods for the delivery of probes into cells. A cyanine-dye labeled oligonucleotide probe containing the new quencher-release linker showed unprecedentedly low background signal and high fluorescence turn-on ratios. The combination of a fluorescein-containing and a near-IR emitting probe discriminated E. coli from S. enterica despite nearly identical ribosomal target sequences. Two-color analysis by microscopy and the first successful discrimination of bacteria by two-color flow cytometry with templated reactive probes are described.
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