ArticleMethods in molecular biology (Clifton, N.J.)2011
Studying G2 DNA damage checkpoints using the fission yeast Schizosaccharomyces pombe.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Cell Cycle Progression and Synchronization: An Overview.Methods in molecular biology (Clifton, N.J.) · 2022Review
- The fission yeast S-phase cyclin Cig2 can drive mitosis.Genetics · 2021Article
- An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells.Frontiers in cell and developmental biology · 2021Article
- Gene Expression of Pneumocystis murina after Treatment with Anidulafungin Results in Strong Signals for Sexual Reproduction, Cell Wall Integrity, and Cell Cycle Arrest, Indicating a Requirement for Ascus Formation for Proliferation.Antimicrobial agents and chemotherapy · 2018Article
- Size-Dependent Expression of the Mitotic Activator Cdc25 Suggests a Mechanism of Size Control in Fission Yeast.Current biology : CB · 2017Article
- Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Using synchronized cells, one can directly measure delay in mitosis brought about by the G2 DNA damage checkpoint in response to exposure to exogenous DNA damaging agents. Scoring mitosis in the fission yeast Schizosaccharomyces pombe is relatively simple. Many techniques exist for synchronizing cells for such assays. We present a detailed explanation of the setup and use of centrifugal elutriation to synchronize cells in G2, exposure of cells to DNA damage, and measurement of mitotic progression and delay.
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Registered trials
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