Evidence map›Paper›PMID 21869468›Full record

ArticleJournal of genetics2011

Intergenic spacer length variants in Old Portuguese bread wheat cultivars.

Ana Carvalho, Henrique Guedes-Pinto, José Lima-Brito

Abstract read
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In one paragraph

Article in Journal of genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.7field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Ana CarvalhoInstitute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology,University of Tras-os-Montes and Alto Douro, PO Box 1013, 5001-801 Vila Real, Portugal.
Henrique Guedes-Pinto
José Lima-Brito
Institute for Biotechnology and Bioengineering · PTUniversity of Trás-os-Montes and Alto Douro · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The intergenic spacer of the ribosomal DNA is highly variable, but is location specific in the nucleolar organizer region of the chromosomes. This study provides an event of high level of polymorphism / size variation and occurrence of 14 unique phenotypes in 48 landraces of Portuguese bread wheat cultivars for IGS-amplified products obtained by PCR-RFLP technique performed with TaqI. The attendant IGS polymorphism has been used to deduce affinities between landraces. Some of the high molecular weight IGS allelic variants were also probed for their chromosomal localization by sequential silver nitrate staining and fluorescence in situ hybridization. However, only the intergenic spacer allelic variant of 3.1 kb could be successfully hybridized, and was observed to be physically located on the chromosome pair 1B in the NOR loci of the cultivar 'Magueija'.

Indexed as

Chromosome MappingChromosomes, PlantDeoxyribonucleases, Type II Site-SpecificDNA, Ribosomal SpacerGenetic VariationIn Situ Hybridization, FluorescencePhylogenyPolymorphism, Restriction Fragment LengthTriticumDeoxyribonucleases, Type II Site-SpecificDNA, Ribosomal SpacerTCGA-specific type II deoxyribonucleases

Identifiers

PMID21869468
OpenAlexW1972821173

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.