ArticleMalaria journal2011
Development and evaluation of a prototype non-woven fabric filter for purification of malaria-infected blood.
Article in Malaria journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 31 citations in OpenAlex.
- Low-volumeMicrobial genomics · 2025Article
- Monitoring Plasmodium vivax resistance to antimalarials: Persisting challenges and future directions.International journal for parasitology. Drugs and drug resistance · 2021Review
- A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells.Stem cell reports · 2021Article
- Rodent Malaria Erythrocyte Preference Assessment by anFrontiers in cellular and infection microbiology · 2021Article
- Molecular Surveillance andFrontiers in cellular and infection microbiology · 2021Article
- Detection and stage classification of Plasmodium falciparum from images of Giemsa stained thin blood films using random forest classifiers.Diagnostic pathology · 2020Article
- On the survival of 48 h Plasmodium vivax Aotus monkey-derived ex vivo cultures: the role of leucocytes filtration and chemically defined lipid concentrate media supplementation.Malaria journal · 2020Article
- Purifying stem cell-derived red blood cells: a high-throughput label-free downstream processing strategy based on microfluidic spiral inertial separation and membrane filtration.Biotechnology and bioengineering · 2020Article
- Ex vivo susceptibilities of Plasmodium vivax isolates from the China-Myanmar border to antimalarial drugs and association with polymorphisms in Pvmdr1 and Pvcrt-o genes.PLoS neglected tropical diseases · 2020Article
- Effects and Mechanism of Action of Artemisinin on Mitochondria of Plasmodium berghei.Chinese journal of integrative medicine · 2020Article
- From marginal to essential: the golden thread between nutrient sensing, medium composition and Plasmodium vivax maturation in in vitro culture.Malaria journal · 2019Review
- Return of chloroquine sensitivity to Africa? Surveillance of African Plasmodium falciparum chloroquine resistance through malaria imported to China.Parasites & vectors · 2017Article
- Further evaluation of the NWF filter for the purification of Plasmodium vivax-infected erythrocytes.Malaria journal · 2017Article
- Ex Vivo Maturation Assay for Testing Antimalarial Sensitivity of Rodent Malaria Parasites.Antimicrobial agents and chemotherapy · 2016Article
- Genomic analysis of local variation and recent evolution in Plasmodium vivax.Nature genetics · 2016Article
- The development of malaria diagnostic techniques: a review of the approaches with focus on dielectrophoretic and magnetophoretic methods.Malaria journal · 2016Review
- UTR introns, antisense RNA and differentially spliced transcripts between Plasmodium yoelii subspecies.Malaria journal · 2016Article
- Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model.PloS one · 2016Article
- Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum-infected whole blood samples.Malaria journal · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMany malaria-related studies depend on infected red blood cells (iRBCs) as fundamental material; however, infected blood samples from human or animal models include leukocytes (white blood cells or WBCs), especially difficult to separate from iRBCs in cases involving Plasmodium vivax. These host WBCs are a source of contamination in biology, immunology and molecular biology studies, requiring their removal. Non-woven fabric (NWF) has the ability to adsorb leukocytes and is already used as filtration material to deplete WBCs for blood transfusion and surgery. The present study describes the development and evaluation of a prototype NWF filter designed for purifying iRBCs from malaria-infected blood.
methodsBlood samples of P. vivax patients were processed separately by NWF filter and CF11 column methods. WBCs and RBCs were counted, parasite density, morphology and developing stage was checked by microscopy, and compared before and after treatment. The viability of filtrated P. vivax parasites was examined by in vitro short-term cultivation.
resultsA total of 15 P. vivax-infected blood samples were treated by both NWF filter and CF11 methods. The WBC removal rate of the NWF filter method was 99.03%, significantly higher than the CF11 methods (98.41%, P < 0.01). The RBC recovery rate of the NWF filter method was 95.48%, also significantly higher than the CF11 method (87.05%, P < 0.01). Fourteen in vitro short-term culture results showed that after filter treatment, P. vivax parasite could develop as normal as CF11 method, and no obvious density, developing stage difference were fund between two methods.
conclusionsNWF filter filtration removed most leukocytes from malaria-infected blood, and the recovery rate of RBCs was higher than with CF11 column method. Filtrated P. vivax parasites were morphologically normal, viable, and suitable for short-term in vitro culture. NWF filter filtration is simple, fast and robust, and is ideal for purification of malaria-infected blood.
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