ArticleMolecular biology of the cell2011
The functionally distinct fission yeast formins have specific actin-assembly properties.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 54 citations in OpenAlex.
- Impact of N-terminal dimerization on formin homology 1 domain polymer dynamics and actin assembly.Biophysical journal · 2026Article
- Impact of N-terminal dimerization on formin homology 1 domain polymer dynamics and actin assembly.bioRxiv : the preprint server for biology · 2025Article
- Article
- Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins.Cytoskeleton (Hoboken, N.J.) · 2023Article
- Condensation of the fusion focus by the intrinsically disordered region of the formin Fus1 is essential for cell-cell fusion.Current biology : CB · 2022Article
- Actin assembly requirements of the formin Fus1 to build the fusion focus.Journal of cell science · 2022Article
- Cooperative bundling by fascin generates actin structures with architectures that depend on filament length.Frontiers in cell and developmental biology · 2022Article
- Nucleation limits the lengths of actin filaments assembled by formin.Biophysical journal · 2021Article
- Negative control of cytokinesis by stress-activated MAPK signaling.Current genetics · 2021Review
- Formin Cdc12's specific actin assembly properties are tailored for cytokinesis in fission yeast.Biophysical journal · 2021Article
- Review
- Profilin's Affinity for Formin Regulates the Availability of Filament Ends for Actin Monomer Binding.Journal of molecular biology · 2020Article
- Article
- Competition for delivery of profilin-actin to barbed ends limits the rate of formin-mediated actin filament elongation.The Journal of biological chemistry · 2020Article
- Article
- Capping Protein Insulates Arp2/3-Assembled Actin Patches from Formins.Current biology : CB · 2019Article
- Article
- Cell Biology: Capturing Formin's Mechano-Inhibition.Current biology : CB · 2017Article
- Direct Visualization and Quantification of the Actin Nucleation andElongation EventsBio-protocol · 2017Article
- Profilin Interaction with Actin Filament Barbed End Controls Dynamic Instability, Capping, Branching, and Motility.Developmental cell · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
Fission yeast expresses three formins required for distinct actin cytoskeletal processes: Cdc12 (cytokinesis), For3 (polarization), and Fus1 (mating). We propose that in addition to differential regulation, key actin-assembly properties tailor formins for a particular role. In direct comparison to the well-studied Cdc12, we report the first in vitro characterization of the actin-assembly properties of For3 and Fus1. All three share fundamental formin activities; however, particular reaction rates vary significantly. Cdc12 is an efficient nucleator (one filament per approximately 3 Cdc12 dimers) that processively elongates profilin-actin at a moderate rate of 10 subunits s(-1) μM(-1), but lacks filament-bundling activity. Fus1 is also an efficient nucleator, yet processively elongates profilin-actin at one-half the rate of and dissociates 10-fold more rapidly than Cdc12; it also bundles filaments. For3 nucleates filaments 100-fold less well than Fus1, but like Cdc12, processively elongates profilin-actin at a moderate rate and lacks filament-bundling activity. Additionally, both the formin homology FH1 and FH2 domains contribute to the overall rate of profilin-actin elongation. We also confirmed the physiological importance of the actin-assembly activity of the fission yeast formins. Point mutants that disrupt their ability to stimulate actin assembly in vitro do not function properly in vivo.
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