ArticlePlant physiology2011
Antisense suppression of the small chloroplast protein CP12 in tobacco alters carbon partitioning and severely restricts growth.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Photosynthetic glyceraldehyde 3-phosphate dehydrogenase B is present in the last common ancestor of Viridiplantae predating the Streptophyta-Chlorophyta split.The New phytologist · 2026Article
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Hidden protein disorder: Deciphering the structural organisation and dynamics of a non-canonical CP12 from the diatom Thalassiosira pseudonana.The FEBS journal · 2025Article
- The impact of CP12 on the metabolome of cyanobacteria under fluctuating COFrontiers in plant science · 2025Article
- Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato.Plants (Basel, Switzerland) · 2022Article
- A Trajectory of Discovery: Metabolic Regulation by the Conditionally Disordered Chloroplast Protein, CP12.Biomolecules · 2022Review
- Reduction in Phosphoribulokinase Amount and Re-Routing Metabolism inInternational journal of molecular sciences · 2022Article
- Flexibility of Oxidized and Reduced States of the Chloroplast Regulatory Protein CP12 in Isolation and in Cell Extracts.Biomolecules · 2021Article
- A new type of flexible CP12 protein in the marine diatom Thalassiosira pseudonana.Cell communication and signaling : CCS · 2021Article
- Feeding the world: improving photosynthetic efficiency for sustainable crop production.Journal of experimental botany · 2019Review
- The Spermine Synthase OsSPMS1 Regulates Seed Germination, Grain Size, and Yield.Plant physiology · 2018Article
- Chloroplast Protein 12 Expression Alters Growth and Chilling Tolerance in Tropical ForageFrontiers in plant science · 2018Article
- Article
- Arabidopsis CP12 mutants have reduced levels of phosphoribulokinase and impaired function of the Calvin-Benson cycle.Journal of experimental botany · 2017Article
- Identification and reproducibility of diagnostic DNA markers for tuber starch and yield optimization in a novel association mapping population of potato (Solanum tuberosum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Fairy "tails": flexibility and function of intrinsically disordered extensions in the photosynthetic world.Frontiers in molecular biosciences · 2015Review
- The CP12 protein family: a thioredoxin-mediated metabolic switch?Frontiers in plant science · 2014Review
- Article
- Redox regulation of the Calvin-Benson cycle: something old, something new.Frontiers in plant science · 2013Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The thioredoxin-regulated chloroplast protein CP12 forms a multienzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK and GAPDH are inactivated when present in this complex, a process shown in vitro to be dependent upon oxidized CP12. The importance of CP12 in vivo in higher plants, however, has not been investigated. Here, antisense suppression of CP12 in tobacco (Nicotiana tabacum) was observed to impact on NAD-induced PRK and GAPDH complex formation but had little effect on enzyme activity. Additionally, only minor changes in photosynthetic carbon fixation were observed. Despite this, antisense plants displayed changes in growth rates and morphology, including dwarfism and reduced apical dominance. The hypothesis that CP12 is essential to separate oxidative pentose phosphate pathway activity from Calvin-Benson cycle activity, as proposed in cyanobacteria, was tested. No evidence was found to support this role in tobacco. Evidence was seen, however, for a restriction to malate valve capacity, with decreases in NADP-malate dehydrogenase activity (but not protein levels) and pyridine nucleotide content. Antisense repression of CP12 also led to significant changes in carbon partitioning, with increased carbon allocation to the cell wall and the organic acids malate and fumarate and decreased allocation to starch and soluble carbohydrates. Severe decreases were also seen in 2-oxoglutarate content, a key indicator of cellular carbon sufficiency. The data presented here indicate that in tobacco, CP12 has a role in redox-mediated regulation of carbon partitioning from the chloroplast and provides strong in vivo evidence that CP12 is required for normal growth and development in plants.
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Registered trials
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