Evidence map›Paper›PMID 21865445›Full record

ArticleThe Journal of general virology2011

Envelope and pre-membrane protein structural amino acid mutations mediate diminished avian growth and virulence of a Mexican West Nile virus isolate.

Stanley A Langevin, Richard A Bowen, Wanichaya N Ramey, Todd A Sanders, Payal D Maharaj, Ying Fang, Jennine Cornelius, Christopher M Barker, William K Reisen, David W C Beasley and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. West Nile viral infection of equids.Veterinary microbiology · 2013
    Review
  11. Review
  12. Article
  13. West Nile virus population genetics and evolution.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012
    Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 1 country.

Stanley A LangevinCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Richard A BowenDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Wanichaya N RameyCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Todd A SandersColorado Division of Wildlife, Fort Collins, CO 80526, USA.
Payal D MaharajCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Ying FangCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Jennine CorneliusCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Christopher M BarkerCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
William K ReisenCenter for Vectorborne Diseases and Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
David W C BeasleyDepartments of Pathology and Microbiology and Immunology, Center for Emerging Infectious Diseases and Biodefense, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555, USA.
Alan D T BarrettDepartments of Pathology and Microbiology and Immunology, Center for Emerging Infectious Diseases and Biodefense, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555, USA.
Richard M KinneyDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
Claire Y-H HuangDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
Aaron C BraultDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.
University of California, Davis · USCenters for Disease Control and Prevention · USThe University of Texas Medical Branch at Galveston · USColorado Parks and Wildlife · USColorado State University · US

Funding

West Nile Viral Determinants of Avian PathogenesisR01AI061822 · NIAID · UNIVERSITY OF CALIFORNIA DAVIS · PI BRAULT, AARON COLE · 2004 to 2007
$1.3M
West Nile Virus Genotypic Affect on Host CompetenceR01CI000235 · CI · UNIVERSITY OF CALIFORNIA DAVIS · PI BRAULT, AARON COLE · 2004 to 2006
$181k
NCPDCID CDC HHS CI000235NCPDCID CDC HHS R01 CI000235NIAID NIH HHS AI061822NIAID NIH HHS AI55606NIAID NIH HHS R01 AI061822
6 · The paper itself

Abstract

The hallmark attribute of North American West Nile virus (WNV) strains has been high pathogenicity in certain bird species. Surprisingly, this avian virulent WNV phenotype has not been observed during its geographical expansion into the Caribbean, Central America and South America. One WNV variant (TM171-03-pp1) isolated in Mexico has demonstrated an attenuated phenotype in two widely distributed North American bird species, American crows (AMCRs) and house sparrows (HOSPs). In order to identify genetic determinants associated with attenuated avian replication of the TM171-03-pp1 variant, chimeric viruses between the NY99 and Mexican strains were generated, and their replicative capacity was assessed in cell culture and in AMCR, HOSP and house finch avian hosts. The results demonstrated that mutations in both the pre-membrane (prM-I141T) and envelope (E-S156P) genes mediated the attenuation phenotype of the WNV TM171-03-pp1 variant in a chicken macrophage cell line and in all three avian species assayed. Inclusion of the prM-I141T and E-S156P TM171-03-pp1 mutations in the NY99 backbone was necessary to achieve the avian attenuation level of the Mexican virus. Furthermore, reciprocal incorporation of both prM-T141I and E-P156S substitutions into the Mexican virus genome was necessary to generate a virus that exhibited avian virulence equivalent to the NY99 virus. These structural changes may indicate the presence of new evolutionary pressures exerted on WNV populations circulating in Latin America or may signify a genetic bottleneck that has constrained their epiornitic potential in alternative geographical locations.

Indexed as

Amino Acid SubstitutionAnimalsBird DiseasesCell LineChickensCloning, MolecularCrowsDNA, ComplementaryFinchesMembrane ProteinsMexicoMutationPhenotypePhylogeographyPlasmidsSequence Analysis, DNADNA, ComplementaryMembrane ProteinsViral Envelope Proteins

Identifiers

PMID21865445
PMCPMC3352571
OpenAlexW1998748437

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.