ArticleJournal of clinical microbiology2011
Rapid multiplexed immunoassay for simultaneous serodiagnosis of HIV-1 and coinfections.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Forty years of advances in optical biosensors-are "autonomous" biosensors in our future?Analytical and bioanalytical chemistry · 2024Review
- Rapid, high-sensitivity detection of biomolecules using dual-comb biosensing.Scientific reports · 2023Article
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- Near patient CD4 count in a hospitalized HIV patient population.Cytometry. Part B, Clinical cytometry · 2017Article
- Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Microarray Immunoassay for Serum Thyrotropin and Thyroglobulin Using Antibodies Immobilized on Track-Etched Membranes.Indian journal of clinical biochemistry : IJCB · 2017Article
- Diagnostic Performance of Tuberculosis-Specific IgG Antibody Profiles in Patients with Presumptive Tuberculosis from Two Continents.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Optical biosensors.Essays in biochemistry · 2016Review
- Evanescent wave fluorescence biosensors: Advances of the last decade.Biosensors & bioelectronics · 2016Review
- Programmable bio-nano-chip system: a flexible point-of-care platform for bioscience and clinical measurements.Lab on a chip · 2015Article
- Factors associated with failure to screen for syphilis during antenatal care in Ghana: a case control study.BMC infectious diseases · 2015Article
- Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 1/2 and Hepatitis C Virus Using Sol-Gel Based Microarray.BioMed research international · 2015Article
- Microfluidic devices for nucleic acid (NA) isolation, isothermal NA amplification, and real-time detection.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Article
- Performance of a multiplexed serological microarray for the detection of antibodies against central nervous system pathogens.Journal of microbiological methods · 2014Article
- Performance evaluation of the MBio Diagnostics point-of-care CD4 counter.Journal of immunological methods · 2013Article
- Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Diagnosis of opportunistic infections in HIV-infected individuals remains a major public health challenge, particularly in resource-limited settings. Here, we describe a rapid diagnostic system that delivers a panel of serologic immunoassay results using a single drop of blood, serum, or plasma. The system consists of disposable cartridges and a simple reader instrument, based on an innovative implementation of planar waveguide imaging technology. The cartridge incorporates a microarray of recombinant antigens and antibody controls in a fluidic channel, providing multiple parallel fluorescence immunoassay results for a single sample. This study demonstrates system performance by delivering antibody (Ab) reactivity results simultaneously for multiple antigens of HIV-1, Treponema pallidum (syphilis), and hepatitis C virus (HCV) in a collection of clinical serum, plasma, and whole-blood samples. By plotting antibody reactivity (fluorescence intensity) for known positive and negative samples, empirical reactivity cutoff values were defined. The HIV-1 assay shows 100% agreement with known seroreactivity for a collection of 82 HIV Ab-positive and 142 HIV Ab-negative samples, including multiple samples with HCV and syphilis coinfection. The treponema-specific syphilis assay correctly identifies 67 of 68 T. pallidum Ab-positive and 100 of 102 T. pallidum Ab-negative samples, and the HCV assay correctly identifies 59 of 60 HCV Ab-positive and 120 of 121 HCV Ab-negative samples. Multiplexed assay performance for whole-blood samples is also demonstrated. The ability to diagnose HIV and opportunistic infections simultaneously at the point of care should lead to more effective therapy decisions and improved linkage to care.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.