ArticleDevelopment (Cambridge, England)2011
Combinatorial activation and concentration-dependent repression of the Drosophila even skipped stripe 3+7 enhancer.
Article in Development (Cambridge, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 54 citations in OpenAlex.
- Compensatory action of different types of cis-regulatory elements buffers the striped expression of Drosophila pair-rule genes.Development (Cambridge, England) · 2025Article
- Multi-level regulation of even-skipped stripes by the ubiquitous factor Zelda.Development (Cambridge, England) · 2023Article
- Transcriptional activators in the early Drosophila embryo perform different kinetic roles.Cell systems · 2023Article
- Mechanisms of Interaction between Enhancers and Promoters in ThreeInternational journal of molecular sciences · 2023Review
- Article
- Repression precedes independent evolutionary gains of a highly specific gene expression pattern.Cell reports · 2021Article
- Quantitative analysis reveals genotype- and domain- specific differences between mRNA and protein expression of segmentation genes in Drosophila.Developmental biology · 2019Article
- Hunchback is counter-repressed to regulate even-skipped stripe 2 expression in Drosophila embryos.PLoS genetics · 2018Article
- Patterns of chromatin accessibility along the anterior-posterior axis in the early Drosophila embryo.PLoS genetics · 2018Article
- A conserved maternal-specific repressive domain in Zelda revealed by Cas9-mediated mutagenesis in Drosophila melanogaster.PLoS genetics · 2017Article
- Synthetic enhancer design by in silico compensatory evolution reveals flexibility and constraint in cis-regulation.BMC systems biology · 2017Article
- Quantitative Measurement and Thermodynamic Modeling of Fused Enhancers Support a Two-Tiered Mechanism for Interpreting Regulatory DNA.Cell reports · 2017Article
- A Looping-Based Model for Quenching Repression.PLoS computational biology · 2017Article
- A sequence level model of an intact locus predicts the location and function of nonadditive enhancers.PloS one · 2017Article
- Spatial distribution of predicted transcription factor binding sites in Drosophila ChIP peaks.Mechanisms of development · 2016Article
- Predicting Ancestral Segmentation Phenotypes from Drosophila to Anopheles Using In Silico Evolution.PLoS genetics · 2016Article
- Zelda is differentially required for chromatin accessibility, transcription factor binding, and gene expression in the early Drosophila embryo.Genome research · 2015Article
- Krüppel Expression Levels Are Maintained through Compensatory Evolution of Shadow Enhancers.Cell reports · 2015Article
- Transcriptional activation is a conserved feature of the early embryonic factor Zelda that requires a cluster of four zinc fingers for DNA binding and a low-complexity activation domain.The Journal of biological chemistry · 2015Article
- Shadow enhancers enable Hunchback bifunctionality in the Drosophila embryo.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Despite years of study, the precise mechanisms that control position-specific gene expression during development are not understood. Here, we analyze an enhancer element from the even skipped (eve) gene, which activates and positions two stripes of expression (stripes 3 and 7) in blastoderm stage Drosophila embryos. Previous genetic studies showed that the JAK-STAT pathway is required for full activation of the enhancer, whereas the gap genes hunchback (hb) and knirps (kni) are required for placement of the boundaries of both stripes. We show that the maternal zinc-finger protein Zelda (Zld) is absolutely required for activation, and present evidence that Zld binds to multiple non-canonical sites. We also use a combination of in vitro binding experiments and bioinformatics analysis to redefine the Kni-binding motif, and mutational analysis and in vivo tests to show that Kni and Hb are dedicated repressors that function by direct DNA binding. These experiments significantly extend our understanding of how the eve enhancer integrates positive and negative transcriptional activities to generate sharp boundaries in the early embryo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.