ArticleJournal of experimental botany2011
Molecular analysis of T-DNA insertion mutants identified putative regulatory elements in the AtTERT gene.
Article in Journal of experimental botany, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 18 citations in OpenAlex.
- Telomerase Interaction Partners-Insight from Plants.International journal of molecular sciences · 2021Article
- Arabidopsis retains vertebrate-type telomerase accessory proteins via a plant-specific assembly.Nucleic acids research · 2021Article
- Telomerase RNAs in land plants.Nucleic acids research · 2019Article
- An armadillo-domain protein participates in a telomerase interaction network.Plant molecular biology · 2018Article
- Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum.Planta · 2017Article
- Transitions between the Arabidopsis-type and the human-type telomere sequence in green algae (clade Caudivolvoxa, Chlamydomonadales).Chromosoma · 2016Article
- Telomere- and Telomerase-Associated Proteins and Their Functions in the Plant Cell.Frontiers in plant science · 2016Review
- cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase.Frontiers in plant science · 2015Article
- Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants.The Plant journal : for cell and molecular biology · 2015Article
- A telomerase-independent component of telomere loss in chromatin assembly factor 1 mutants of Arabidopsis thaliana.Chromosoma · 2013Article
- T-DNA insertion mutants reveal complex expression patterns of the aldehyde dehydrogenase 3H1 locus in Arabidopsis thaliana.Journal of experimental botany · 2012Article
- Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment.Journal of experimental botany · 2012Article
- Three TERT genes in Nicotiana tabacum.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
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Authors and funding
6 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Analysis of plants bearing a T-DNA insertion is a potent tool of modern molecular biology, providing valuable information about the function and involvement of genes in metabolic pathways. A collection of 12 Arabidopsis thaliana lines with T-DNA insertions in the gene coding for the catalytic subunit of telomerase (AtTERT) and in adjacent regions was screened for telomerase activity [telomere repeat amplification protocol (TRAP) assay], telomere length (terminal restriction fragments), and AtTERT transcription (quantitative reverse transcription-PCR). Lines with the insertion located upstream of the start codon displayed unchanged telomere stability and telomerase activity, defining a putative minimal AtTERT promoter and the presence of a regulatory element linked to increased transcription in the line SALK_048471. Lines bearing a T-DNA insertion inside the protein-coding region showed telomere shortening and lack of telomerase activity. Transcription in most of these lines was unchanged upstream of the T-DNA insertion, while it was notably decreased downstream. The expression profile varied markedly in mutant lines harbouring insertions at the 5' end of AtTERT which showed increased transcription and abolished tissue specificity. Moreover, the line FLAG_385G01 (T-DNA insertion inside intron 1) revealed the presence of a highly abundant downstream transcript with normal splicing but without active telomerase. The role of regulatory elements found along the AtTERT gene is discussed in respect to natural telomerase expression and putative intron-mediated enhancement.
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